This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ BRAF / B-Raf Antibody (clone 1H12F1) LS-B3444
catalog :
LS-B3444
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
1H12F1

The same clone is also sold as:
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section
product information
AntibodyID :
106117
AntibodyName :
LS-B3444
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IHC-plus™ BRAF / B-Raf Antibody (clone 1H12F1) LS-B3444
Specificity :
Ni-NTA purified truncated recombinant BRAF expressed in E. coli strain BL21 (DE3).
ClonalityDesc :
Monoclonal
CloneName :
1H12F1
AntibodyModification :
Unconjugated
PresentationDesc :
Ascitic fluid, 0.03% sodium azide
ImmunogenType :
Recombinant protein
ImmunogenDesc :
Purified recombinant fragment of BRAF expressed in E. coli.
PurificationDesc :
Ascites
RecommendedStorageDesc :
Short term: store at 4°C. Long term: store at -20°C.
IsotypeName :
IgG1
Gene :
BRAF / B-Raf
StandardGeneSymbol :
BRAF
gene family :
Protein Kinase
Subfamily :
Raf
Reactivity :
Human
Usage :
ELISA (1:10000), IHC, IHC-P (1:200), WB (1:100 - 1:500)
ShortWebDescription :
B-Raf antibody LS-B3444 is an unconjugated mouse monoclonal antibody to human B-Raf (BRAF). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
UsageText :
Immunohistochemistry: LS-B3444 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3444 was determined to be 1:200.
Synonyms :
BRAF, 94 kDa B-raf protein, B-raf, BRAF1, B Raf, Proto-oncogene B-Raf, p94, B-RAF1, NS7, ONCOGENE BRAF, RAFB1
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.