product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ PARP1 Antibody (clone A6.4.12) LS-B3432
catalog :
LS-B3432
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
A6.4.12
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Published Application/Species/Sample/DilutionReference
  • western blot; human; loading ...; fig 5c, 7e
Zheng J, Croteau D, Bohr V, Akbari M. Diminished OPA1 expression and impaired mitochondrial morphology and homeostasis in Aprataxin-deficient cells. Nucleic Acids Res. 2019;: pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B3432 image 1
Anti-PARP antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
product information
AntibodyID :
106104
AntibodyName :
LS-B3432
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IHC-plus™ PARP1 Antibody (clone A6.4.12) LS-B3432
Specificity :
Recognizes poly (ADP-ribose) polymerase 1 (PARP-1), a ~116kD nuclear enzyme cleaved during apoptosis. PARP-1, a caretaker enzyme, is involved in DNA damage repair, plays roles in diabetes pathophysiology and tumor proliferation.). As well as protecting cells from genomic instability, PARP-1 is involved in the development of both inflammatory and immune responses, and cell death by apoptosis and necrosis. Targets PARP-1, an enzyme which represents a promising target for new developments in therapeutic treatment of immune mediated diseases. PARP-1 has considerable potential for delivering selective tumor cell killing while sparing normal cells.
ClonalityDesc :
Monoclonal
CloneName :
A6.4.12
AntibodyModification :
Unconjugated
PresentationDesc :
PBS, 0.09% Sodium Azide
ImmunogenType :
Purified protein
ImmunogenDesc :
Human PARP.
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
IsotypeName :
IgG1
Gene :
PARP1
StandardGeneSymbol :
PARP1
Reactivity :
Human
Usage :
ELISA (1:1000 - 1:5000), IF, IHC, IHC-Fr, IHC-P (10 µg/ml), IP, WB
ShortWebDescription :
PARP1 antibody LS-B3432 is an unconjugated mouse monoclonal antibody to human PARP1. Validated for ELISA, IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
UsageText :
Immunohistochemistry: LS-B3432 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3432 was determined to be 10 ug/ml.
Synonyms :
PARP1, ADPRT, ADP-ribosyltransferase NAD(+), Adp-ribosyltransferase, ADPRT1, ARTD1, ADPRT 1, Poly(ADP-ribose) synthetase, Poly(ADP-ribosyl)transferase, PARP, Poly [ADP-ribose] polymerase 1, PADPRT-1, PARP-1, Poly (ADP-ribose) polymerase 1, Poly(ADP-ribose) polymerase, Poly[ADP-ribose] synthase 1, PPOL
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.