product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ Collagen VI Antibody LS-B342
catalog :
LS-B342
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Reference
Wu X, Serna V, Thomas J, Qiang W, Blumenfeld M, Kurita T. Subtype-Specific Tumor-Associated Fibroblasts Contribute to the Pathogenesis of Uterine Leiomyoma. Cancer Res. 2017;77:6891-6901 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B342 image 1
Anti-Collagen I antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
product information
AntibodyID :
31301
AntibodyName :
LS-B342
TargetSpecies :
Mammal
Host Species :
Rabbit
Product Name :
IHC-plus™ Collagen VI Antibody LS-B342
Specificity :
Typically negligible cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with human, bovine, and most mammalian Type I collagens with negligible cross-reactivity with Type II, III, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
PresentationDesc :
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
ImmunogenType :
Purified protein
ImmunogenDesc :
Collagen Type I from human and bovine placenta.
PurificationDesc :
Affinity chromatography
RecommendedStorageDesc :
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG
Gene :
Collagen VI
Reactivity :
Bovine, Mammal, Human
Usage :
ELISA (1:5000 - 1:50000), FLISA (1:100), IHC, IHC-P (2.5 µg/ml), IP (1:100), WB (1:1000 - 1:10000)
ShortWebDescription :
Collagen VI antibody LS-B342 is an unconjugated rabbit polyclonal antibody to Collagen VI from mammal. It is reactive with human, bovine and mammal. Validated for ELISA, FLISA, IHC, IP and WB. Cited in 5 publications.
UsageText :
Immunohistochemistry: LS-B342 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B342 was determined to be 2.5 ug/ml.
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.