This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
PathPlus™ HSPB1 / HSP27 Antibody (clone 5F12-B10) LS-B3412
catalog :
LS-B3412
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
5F12-B10
reactivity :
human
application :
western blot, immunohistochemistry, immunohistochemistry - paraffin section
product information
AntibodyID :
106060
AntibodyName :
LS-B3412
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
PathPlus™ HSPB1 / HSP27 Antibody (clone 5F12-B10) LS-B3412
ClonalityDesc :
Monoclonal
CloneName :
5F12-B10
AntibodyModification :
Unconjugated
PresentationDesc :
PBS, pH 7.2, 0.09% azide, 50% glycerol
ImmunogenDesc :
Native human Hsp27 protein.
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
IsotypeName :
IgG1
Gene :
HSPB1 / HSP27
StandardGeneSymbol :
HSPB1
Reactivity :
Human, Monkey
Usage :
IHC, IHC-P (10 µg/ml), WB (1:1000)
ShortWebDescription :
HSP27 antibody LS-B3412 is an unconjugated mouse monoclonal antibody to HSP27 (HSPB1 ) from human and monkey. Validated for IHC and WB.
UsageText :
Immunohistochemistry: LS-B3412 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3412 was determined to be 10 ug/ml.
Synonyms :
HSPB1, 28 kDa heat shock protein, Heat shock 27 kDa protein, Heat shock 27kD protein 1, Heat shock 27kDa protein 1, HSP27, HSP28, HSP 27, Hsp25, SRP27, Stress-responsive protein 27, CMT2F, Heat shock 27 kd protein, Heat shock protein beta-1, HMN2B, HS.76067
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
antibodies@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.