This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ SOCS3 Antibody (clone SO1) LS-B3317
catalog :
LS-B3317
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
SO1

The same clone is also sold as:
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section
product information
AntibodyID :
104578
AntibodyName :
LS-B3317
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IHC-plus™ SOCS3 Antibody (clone SO1) LS-B3317
Specificity :
Recognizes suppressor of cytokine signaling 3 (SOCS3), a 30kD member of the suppressor of cytokine signaling family (SOCS) which negatively regulate Janus Kinase (JAK) and signal transducer and activators of transcription (STAT) signaling pathways. SOCS3 specifically acts as a negative feedback regulator for the STAT3 pathways, by directly binding to the Tyr-759 motif of the IL-6 signal-transducing receptor subunit gp130, thereby preventing the IL-6 activation of STAT3. The expression of SOCS3 is inducible by numerous factors, including IL-6, IL-10, Toll-like receptor (TLR) agonists and IFN gamma, making this protein the focus of research into inflammatory diseases, such as Arthritis, Colitis, and Crohn's Disease. SOCS3 expression has also been shown to affect IFN alpha sensitivity in tumor studies.
ClonalityDesc :
Monoclonal
CloneName :
SO1
AntibodyModification :
Unconjugated
PresentationDesc :
PBS, 0.09% Sodium Azide
ImmunogenType :
Recombinant protein
ImmunogenDesc :
Full - length SOCS3 protein.
PurificationDesc :
Protein A purified
RecommendedStorageDesc :
Store at 4°C or at -20°C. Store undiluted. Avoid freeze/thaw cycles.
IsotypeName :
IgG2b
Gene :
SOCS3
StandardGeneSymbol :
SOCS3
Reactivity :
Human
Usage :
ELISA (1:100 - 1:500), IHC, IHC-P (10 µg/ml), WB
ShortWebDescription :
SOCS3 antibody LS-B3317 is an unconjugated mouse monoclonal antibody to human SOCS3. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
UsageText :
Immunohistochemistry: LS-B3317 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3317 was determined to be 10 ug/ml. Western Blot: LS-B3317 detects a band of approximately 30kD in Hela cell lysates.
Synonyms :
SOCS3, Cish3, Cytokine-induced SH2 protein 3, CIS-3, CIS3, SSI-3, STAT-induced STAT inhibitor 3, SSI3, ATOD4, SOCS-3
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.