This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ MAdCAM-1 Antibody (clone 314G8, Low Endotoxin) LS-B3316
catalog :
LS-B3316
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
314G8

The same clone is also sold as:
reactivity :
human
application :
immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
product information
AntibodyID :
104577
AntibodyName :
LS-B3316
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IHC-plus™ MAdCAM-1 Antibody (clone 314G8, Low Endotoxin) LS-B3316
Specificity :
Recognizes human mucosal addressin cell adhesion molecule 1 (MAdCAM-1), a 60kD cell surface protein that is involved in lymphocyte trafficking. MAdCAM-1 is expressed on high endothelial venules of Peyers patches and mesenteric lymph nodes. MAdCAM-1 expression has also been reported in the gut lamina propria but clone 314G8 does not recognize MAdCAM-1 in these tissues. Clone Reacts with the ligand-binding first Ig domain and reports suggest that a splice variant exists in the gut which is not recognized by clone 314G8. Clone 314G8 is reported to block the interaction of MAdCAM-1 with alpha 4 beta 7.
ClonalityDesc :
Monoclonal
CloneName :
314G8
AntibodyModification :
Low Endotoxin Unconjugated
PresentationDesc :
PBS
ImmunogenType :
Recombinant protein
ImmunogenDesc :
Recombinant soluble MAdCAM-1-fc fusion protein
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
Store at -20°C. Store undiluted. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
IsotypeName :
IgG1
Gene :
MAdCAM-1
StandardGeneSymbol :
MADCAM1
Reactivity :
Human
Usage :
ELISA, Flo (1:10 - 1:100), IHC, IHC-Fr (1:25 - 1:200), IHC-P (10 µg/ml)
ShortWebDescription :
MAdCAM-1 antibody LS-B3316 is an unconjugated mouse monoclonal antibody to human MAdCAM-1. Validated for ELISA, Flow and IHC. Tested on 20 paraffin-embedded human tissues.
UsageText :
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100ul. Western Blot: LS-B3316 detects a band of approximately 60kD. Reducing conditions required. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
Synonyms :
MADCAM1, HMAdCAM-1, MACAM1, MAdCAM-1
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.