This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
BAD Antibody (N-Terminus, clone BYC001) IHC-plus™ LS-B3293
catalog :
LS-B3293
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
BYC001
reactivity :
human, mouse
application :
western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section
product information
?AntibodyID :
104554
AntibodyName :
LS-B3293
TargetSpecies :
Mouse
Host Species :
Mouse
Product Name :
BAD Antibody (N-Terminus, clone BYC001) IHC-plus™ LS-B3293
Specificity :
Recognizes human Bad, Mr 23kD, and mouse Bad, Mr 31kD. Occasionally a Mr 14kD protein is detected in human lysates.
ClonalityDesc :
Monoclonal
CloneName :
BYC001
AntibodyModification :
Unconjugated
AntigenModification :
N-Terminus
PresentationDesc :
0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide. Sourced in Ascites.
ImmunogenType :
Recombinant protein
ImmunogenDesc :
Full-length murine Bad containing a N-terminal His6-tag.
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG
Gene :
BAD
StandardGeneSymbol :
BAD
gene family :
Apoptosis
Reactivity :
Mouse, Human
Usage :
IHC, IHC-P (10 µg/ml), IP, WB
UsageText :
Immunohistochemistry: LS-B3293 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3293 was determined to be 10 ug/ml. Western Blot: Proteins were visualized using IgG (HRP) goat anti-mouse and a chemiluminescence detection system. The endogenous level of Bad in most lysates is below the detectable limits of this antibody. Immunoprecipitation: 4 ug immunoprecipitates Bad which was subsequently detected using anti-phospho Bad.
Synonyms :
BAD, BBC2, Bcl-2-like protein 8, BCL2-binding component 6, BCL2L8, BBC6, BCL-X/BCL-2 binding protein, Bcl-2-binding component 6, Bcl2 antagonist of cell death, BCL2-binding protein, Bcl2-L-8
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
antibodies@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.