This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ H2AFX / H2AX Antibody (C-Terminus) LS-B3282
catalog :
LS-B3282
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
product information
AntibodyID :
104540
AntibodyName :
LS-B3282
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ H2AFX / H2AX Antibody (C-Terminus) LS-B3282
Specificity :
Recognizes human histone H2AX. H2AX comprises approximately 2-25% of the histone H2A pool in mammalian cells. The extended C-terminal tail of H2AX contains a serine (Ser139) embedded in an conserved SQE motif, which is rapidly phosphorylated at sites of double strand breaks and stalled replication forks. Phosphorylated H2AX may serve to recruit other repair proteins to the site of DNA damage. H2AX has been implicated in both homologous recombination and nonhomologous end joining DNA repair pathways.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
C-Terminus
PresentationDesc :
Tris-citrate/phosphate, 0.09% sodium azide.
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Synthetic peptide corresponding to a region within the C-Terminus of human H2AX.
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Store at 4°C or -20°C. Avoid freeze-thaw cycles. Store undiluted.
IsotypeName :
IgG
Gene :
H2AFX / H2AX
StandardGeneSymbol :
H2AFX
Reactivity :
Human
Usage :
IF (1:400 - 1:800), IHC, IHC-P (2.5 µg/ml), WB
ShortWebDescription :
H2AX antibody LS-B3282 is an unconjugated rabbit polyclonal antibody to human H2AX (H2AFX) (C-Terminus). Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
UsageText :
Immunohistochemistry: LS-B3282 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3282 was determined to be 2.5 ug/ml.
Synonyms :
H2AFX, H2AX, Histone H2A.x, H2A.X, H2A histone family, member X, H2AX histone
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.