product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ NOVA1 Antibody (aa121-134) LS-B3259
catalog :
LS-B3259
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Published Application/Species/Sample/DilutionReference
  • western blot; human
Zhang Y, Liu H, Zhu J, Zhang D, Shen X, Liu T. RNA binding protein Nova1 promotes tumor growth in vivo and its potential mechanism as an oncogene may due to its interaction with GABAA Receptor-?2. J Biomed Sci. 2016;23:71 pubmed
image
image 1 :
LifeSpan Biosciences LS-B3259 image 1
HEK293 overexpressing NOVA1 (RC210407) and probed with (mock transfection in first lane).
image 2 :
LifeSpan Biosciences LS-B3259 image 2
Anti-NOVA1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
product information
AntibodyID :
102970
AntibodyName :
LS-B3259
TargetSpecies :
Human
Host Species :
Goat
Product Name :
IHC-plus™ NOVA1 Antibody (aa121-134) LS-B3259
Specificity :
Human NOVA1. This antibody is expected to recognise all three reported isoforms (NP_002506.2 ; NP_006480.2 ; NP_006482.1)
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa121-134
PresentationDesc :
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
ImmunogenDesc :
Peptide with sequence C-REMPQNVAKTEPVS, from the internal region of the protein sequence according to NP_002506.2 NP_006480.2 NP_006482.1.
PurificationDesc :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
RecommendedStorageDesc :
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Gene :
NOVA1
BlockingPeptide :
LS-E26643
StandardGeneSymbol :
NOVA1
Reactivity :
Xenopus, Chicken, Bovine, Rabbit, Mouse, Dog, Rat, Pig, Bat, Human, Monkey
Usage :
IHC, IHC-P (2.5 µg/ml), Peptide-ELISA (1:64000), WB (0.01 - 0.03 µg/ml)
ShortWebDescription :
NOVA1 antibody LS-B3259 is an unconjugated goat polyclonal antibody to NOVA1 (aa121-134) from human. It is reactive with human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
UsageText :
Immunohistochemistry: LS-B3259 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3259 was determined to be 2.5 ug/ml.
Synonyms :
NOVA1, Nova-1, Onconeural ventral antigen 1, RNA-binding protein Nova-1, Paraneoplastic Ri antigen
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.