product summary
Loading...
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ IRF6 Antibody (aa455-467) LS-B3231
catalog :
LS-B3231
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, immunohistochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 2
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - paraffin section; mouse; 1:200; loading ...
Tamasas B, Cox T. Massively Increased Caries Susceptibility in an Irf6 Cleft Lip/Palate Model. J Dent Res. 2017;96:315-322 pubmed publisher
Chu E, Tamasas B, Fong H, Foster B, LaCourse M, Tran A, et al. Full Spectrum of Postnatal Tooth Phenotypes in a Novel Irf6 Cleft Lip Model. J Dent Res. 2016;95:1265-73 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B3231 image 1
HEK293 overexpressing IRF6 (RC201579) and probed with (mock transfection in first lane).
image 2 :
LifeSpan Biosciences LS-B3231 image 2
Anti-IRF6 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
product information
AntibodyID :
102942
AntibodyName :
LS-B3231
TargetSpecies :
Human
Host Species :
Goat
Product Name :
IHC-plus™ IRF6 Antibody (aa455-467) LS-B3231
Specificity :
Human IRF6.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa455-467
PresentationDesc :
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
ImmunogenDesc :
Peptide with sequence C-TPSMQLPPALPPQ, from the C-Terminus of protein sequence according to NP_006138.1.
PurificationDesc :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
RecommendedStorageDesc :
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Gene :
IRF6
BlockingPeptide :
LS-E25340
StandardGeneSymbol :
IRF6
Reactivity :
Human
Usage :
IHC, IHC-P (2.5 µg/ml), Peptide-ELISA (1:32000), WB (0.2 - 0.6 µg/ml)
ShortWebDescription :
IRF6 antibody LS-B3231 is an unconjugated goat polyclonal antibody to human IRF6 (aa455-467). Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
UsageText :
Immunohistochemistry: LS-B3231 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3231 was determined to be 2.5 ug/ml.
Synonyms :
IRF6, Interferon regulatory factor 6, OFC6, PPS1, VWS, VWS1, IRF-6, LPS, PIT, PPS, Van der Woude syndrome
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.