This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ ACTB / Beta Actin Antibody (aa350-375) LS-B323
catalog :
LS-B323
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
product information
AntibodyID :
31282
AntibodyName :
LS-B323
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ ACTB / Beta Actin Antibody (aa350-375) LS-B323
Specificity :
Reacts with Human, Rat, Monkey and Mouse. beta-Actin Loading Control Antibody is expected to cross-react with a wide range of species due to sequence homology. Anti-Beta Actin is affinity-purified antibody is directed against human beta Actin protein. A BLAST analysis was used to suggest that this antibody would react with beta Actin from a wide range of organisms, including most vertebrates and some yeast. Broad reactivity makes this antibody an excellent loading control.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa350-375
PresentationDesc :
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Beta-Actin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to C-Terminal region near amino acids 350-375 of Human beta Actin.
PurificationDesc :
Affinity purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
IsotypeName :
IgG
Gene :
ACTB / Beta Actin
StandardGeneSymbol :
ACTB
Reactivity :
Mouse, Goat, Guinea pig, Sheep, Bovine, Rat, Hamster, Pig, Human, Monkey
Usage :
ELISA (1:10000 - 1:40000), IF (1:500 - 1:2000), IHC, IHC-P (5 µg/ml), WB (1:1000 - 1:4000)
ShortWebDescription :
Beta Actin antibody LS-B323 is an unconjugated rabbit polyclonal antibody to Beta Actin (ACTB) (aa350-375) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
UsageText :
Immunohistochemistry: LS-B323 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B323 was determined to be 5 ug/ml.Antibody has been tested in IHC with human and frog samples and in Western blot with human and mouse samples. It is expected to react with a wide range of species due to sequence homology.
Synonyms :
ACTB, Actin, cytoplasmic 1, Beta-actin, Beta actin, BRWS1, Actin, beta, Beta cytoskeletal actin, PS1TP5-binding protein 1, PS1TP5BP1
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.