This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ FHIT Antibody LS-B3166
catalog :
LS-B3166
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section
product information
AntibodyID :
97977
AntibodyName :
LS-B3166
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ FHIT Antibody LS-B3166
Specificity :
Detects full length (~17kD) FHIT protein in cell and tissue lysates derived from human and rat in Western blot. It is not reactive with mouse FHIT protein. Reactivity has been confirmed by Western blotting using lysates from rat brain, colon, spleen, stomach, kidney, lung, small intestine and testes as well as 293T cells and COS cells transfected with a pCMV-FHIT-FLAG construct. Reacts with human and rat FHIT. Cross-reactivity with rat has been confirmed in WB only. Suitability for use with rat in IHC has not yet been evaluated.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
PresentationDesc :
PBS, pH 7.4, 0.1% Sodium Azide
ImmunogenType :
Fusion protein
ImmunogenDesc :
Full length human FHIT fusion protein with GST (glutathione amino transferase)
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
IsotypeName :
IgG
Gene :
FHIT
StandardGeneSymbol :
FHIT
Reactivity :
Rat, Human
Usage :
ELISA, IHC, IHC-P (5 µg/ml), WB
ShortWebDescription :
FHIT antibody LS-B3166 is an unconjugated rabbit polyclonal antibody to FHIT from human. It is reactive with human and rat. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
UsageText :
Immunohistochemistry: LS-B3166 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3166 was determined to be 5 ug/ml.
Synonyms :
FHIT, AP3Aase, Dinucleosidetriphosphatase, Fragile histidine triad, Fragile histidine triad gene, AP3A hydrolase, Bis(5-adenosyl)-triphosphatase, FRA3B, Tumor suppressor protein
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.