product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ Basigin / Emmprin / CD147 Antibody LS-B3163
catalog :
LS-B3163
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
reactivity :
human
application :
immunohistochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; human; 1:500; fig 2
Panchabhai S, Kelemen K, Ahmann G, Sebastian S, Mantei J, Fonseca R. Tumor-associated macrophages and extracellular matrix metalloproteinase inducer in prognosis of multiple myeloma. Leukemia. 2016;30:951-4 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B3163 image 1
Anti-CD147 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
product information
AntibodyID :
97974
AntibodyName :
LS-B3163
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IHC-plus™ Basigin / Emmprin / CD147 Antibody LS-B3163
Specificity :
Recognizes the human CD147 molecule of about 35-40kD.
ClonalityDesc :
Monoclonal
AntibodyModification :
Unconjugated
PresentationDesc :
50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride, 0.5mg/ml Gentamicin
ImmunogenType :
Cells
ImmunogenDesc :
Human T cell line Molt 13
PurificationDesc :
Protein A purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG1,k
Gene :
Basigin / Emmprin / CD147
StandardGeneSymbol :
BSG
Reactivity :
Human
Usage :
Flo, IHC, IHC-Fr, IHC-P (10 µg/ml), IP
ShortWebDescription :
CD147 antibody LS-B3163 is an unconjugated mouse monoclonal antibody to human CD147 (Basigin / Emmprin). Validated for Flow, IHC and IP. Tested on 20 paraffin-embedded human tissues.
UsageText :
Immunohistochemistry: LS-B3163 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3163 was determined to be 10 ug/ml.
Synonyms :
BSG, 5F7, Basigin, Basigin (Ok blood group), CD147 antigen, EMMPRIN, OK, OK blood group antigen, TCSF, CD147, M6
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.