IHC-plus™ CENPF / CENP-F Antibody (aa1759-2093) LS-B3157 | LifeSpan Biosciences LS-B3157 product information

product summary

company name :

LifeSpan Biosciences

product type :

antibody

product name :

IHC-plus™ CENPF / CENP-F Antibody (aa1759-2093) LS-B3157

catalog :

LS-B3157

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

LifeSpan Biosciences product webpage

citations: 1

Published Application/Species/Sample/Dilution	Reference
ELISA; human	Welner S, Trier N, Houen G, Hansen P. Identification and mapping of a linear epitope of centromere protein F using monoclonal antibodies. J Pept Sci. 2013;19:95-101 pubmed publisher

image

image 1 :

Anti-CENPF antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

product information

AntibodyID :

97968

AntibodyName :

LS-B3157

TargetSpecies :

Human

Host Species :

Mouse

Product Name :

IHC-plus™ CENPF / CENP-F Antibody (aa1759-2093) LS-B3157

Specificity :

Recognizes mitosin, a 350 kD phosphoprotein that is specifically involved in mitotic-phase progression. Mitosin is expressed throughout S, G2, and M phases of the cell cycle, but is absent in G0 and G1. It associates with the mitotic apparatus during M-phase and is redistributed from the nucleus to the centromere, spindle, and midbody during M-phase progression. Its usefulness as a proliferation marker is under active investigation.

ClonalityDesc :

Monoclonal

AntibodyModification :

Unconjugated

AntigenModification :

aa1759-2093

PresentationDesc :

PBS, pH 7.4

PurificationDesc :

Protein G purified

RecommendedStorageDesc :

Store at -70°C for up to 1 year. Avoid freeze-thaw cycles.

IsotypeName :

IgG2a,k

Gene :

CENPF / CENP-F

StandardGeneSymbol :

CENPF

Reactivity :

Human

Usage :

IHC, IHC-Fr (10 µg/ml), IHC-P (10 µg/ml), IP (1 - 10 µg/ml), WB (1 - 10 µg/ml)

ShortWebDescription :

CENP-F antibody LS-B3157 is an unconjugated mouse monoclonal antibody to human CENP-F (CENPF) (aa1759-2093). Validated for IHC, IP and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.

UsageText :

Immunohistochemistry: LS-B3157 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3157 was determined to be 10 ug/ml. Western Blot: 1-10 ug/ml. Immunoprecipitation: 1-10 ug/ml. Immunohistochemistry: 10 ug/ml with frozen or paraffin-embedded sections. Tested in IHC using formalin-fixed paraffin-embedded human tissues, HIER antigen retrieval using sodium citrate buffer at pH 6.0.

Synonyms :

CENPF, AH antigen, Centromere protein F, CENF, CENP-F, Kinetochore protein CENPF, Mitosin, CENP-F kinetochore protein, Hcp-1, PRO1779

SalesRegion :

Worldwide

more info or order :

LifeSpan Biosciences product webpage

company information

LifeSpan Biosciences

2401 Fourth Avenue, Suite 900
Seattle, WA 98121

CustomerSupport@lsbio.com

https://www.lsbio.com

1-206-464-1554

headquarters: USA

Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.