This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ FceR1 / Fc Epsilon Receptor 1 Antibody (clone 9E1) LS-B3150
catalog :
LS-B3150
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
9E1
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
product information
AntibodyID :
97961
AntibodyName :
LS-B3150
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IHC-plus™ FceR1 / Fc Epsilon Receptor 1 Antibody (clone 9E1) LS-B3150
Specificity :
Recognizes the human high affinity Fc receptor for IgE (Fc epsilon R1), which plays a central role in the IgE-mediated allergic response. Fc epsilon R1 consists of four subunits, a high affinity IgE binding alpha subunit, a beta chain and two disulfide linked gamma subunits. Clone Recognizes the extracellular D1 domain of the Fc epsilon R1 alpha chain. Fc epsilon R1 is primarily expressed on mast cells and basophils but expression of Fc epsilon R1 has also been reported on monocytes, Langerhans cells and dendritic cells from patients with atopic diseases.
ClonalityDesc :
Monoclonal
CloneName :
9E1
AntibodyModification :
Unconjugated
PresentationDesc :
PBS, 0.09% Sodium Azide
ImmunogenType :
Recombinant protein
PurificationDesc :
Affinity purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG2b
Gene :
FceR1 / Fc Epsilon Receptor 1
StandardGeneSymbol :
FceR1
Reactivity :
Human
Usage :
ELISA, Flo (1:25 - 1:50), IF, IHC, IHC-P (10 µg/ml), WB
ShortWebDescription :
Fc Epsilon Receptor 1 antibody LS-B3150 is an unconjugated mouse monoclonal antibody to human Fc Epsilon Receptor 1 (FceR1). Validated for ELISA, Flow, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
UsageText :
Immunohistochemistry: LS-B3150 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3150 was determined to be 10 ug/ml. Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul.
Synonyms :
, FceR1
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.