This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ LAMP2 / CD107b Antibody LS-B3144
catalog :
LS-B3144
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
reactivity :
human, dogs
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
citations: 2
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; dogs
Furukawa S, Nagaike M, Ozaki K. Databases for technical aspects of immunohistochemistry. J Toxicol Pathol. 2017;30:79-107 pubmed publisher
  • immunohistochemistry; dogs; loading ...; fig 4f
Kyöstilä K, Syrjä P, Jagannathan V, Chandrasekar G, Jokinen T, Seppälä E, et al. A missense change in the ATG4D gene links aberrant autophagy to a neurodegenerative vacuolar storage disease. PLoS Genet. 2015;11:e1005169 pubmed publisher
product information
AntibodyID :
97955
AntibodyName :
LS-B3144
TargetSpecies :
Dog
Host Species :
Mouse
Product Name :
IHC-plus™ LAMP2 / CD107b Antibody LS-B3144
Specificity :
Recognizes canine CD107b, otherwise known as LAMP-2, a 95kD heavily-glycosylated type I integral lysosomal glycoprotein, shown to share high N-terminal amino acid sequence homology with human, mouse and rat CD107b. Species sequence homology: human, mink. Does not react with mouse or rat.
ClonalityDesc :
Monoclonal
AntibodyModification :
Unconjugated
PresentationDesc :
PBS, pH 7.4, 0.09% Sodium Azide
ImmunogenType :
Cells
ImmunogenDesc :
MDCK (Madin-Darby Canine Kidney) cells.
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG1
Gene :
LAMP2 / CD107b
StandardGeneSymbol :
LAMP2
gene family :
Cancer
Reactivity :
Dog, Human
Usage :
Flo, IF, IHC, IHC-P (10 µg/ml), IP, WB
ShortWebDescription :
CD107b antibody LS-B3144 is an unconjugated mouse monoclonal antibody to CD107b (LAMP2) from dog. It is reactive with human and dog. Validated for Flow, IF, IHC, IP and WB. Cited in 2 publications.
UsageText :
Immunohistochemistry: LS-B3144 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3144 was determined to be 10 ug/ml. Flow Cytometry: 10 ul labels 1x10^6 cells in 100ul. Membrane permeabilization is required for this application.
Synonyms :
LAMP2, CD107b, CD107b antigen, LAMPB, LGP110, LAMP-2
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
antibodies@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.