product summary
request information :
company name :
LifeSpan Biosciences
product type :
antibody
product name :
Anti-ACTB / Beta Actin Antibody (N-Terminus, FITC) IHC-plus™ LS-B3143
catalog :
LS-B3143
clonality :
monoclonal
host :
mouse
conjugate :
FITC
reactivity :
human
application :
ELISA, western blot, immunohistochemistry - paraffin section, immunocytochemistry
more info or order :
LifeSpan Biosciences product webpage
image
image 1 :
LifeSpan Biosciences LS-B3143 image 1
Human Prostate (formalin-fixed, paraffin-embedded) stained with ACTB antibody LS-B3143 at 1:50 followed by biotinylated anti-mouse IgG secondary antibody LS-D2, alkaline phosphatase-streptavidin and chromogen.
product information
?AntibodyID :
97954
AntibodyName :
LS-B3143
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
Anti-ACTB / Beta Actin Antibody (N-Terminus, FITC) IHC-plus™ LS-B3143
Specificity :
Recognizes human Beta-Actin.
ClonalityDesc :
Monoclonal
AntibodyModification :
FITC
AntigenModification :
N-Terminus
PresentationDesc :
Lyophilized from PBS, 0.05% sodium azide.
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Synthetic peptide ~ N-terminal, 15-aa corresponding to of human beta-actin (non-muscle) (KLH).
PurificationDesc :
Purified
RecommendedStorageDesc :
+4°C or -20°C, Avoid repeated freezing and thawing.
ResuspensionLiquidDesc :
Reconstitute in 100 ul of Sterile PBS.
IsotypeName :
IgG
Gene :
ACTB / Beta Actin
StandardGeneSymbol :
ACTB
Reactivity :
Human
Usage :
ELISA, IF, IHC-P (1:50), WB
UsageText :
Immunohistochemistry: LS-B3143 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3143 was determined to be 1:50.
Synonyms :
ACTB, Actin, cytoplasmic 1, Beta-actin, Beta actin, BRWS1, Actin, beta, Beta cytoskeletal actin, PS1TP5-binding protein 1, PS1TP5BP1
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
antibodies@lsbio.com
www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.