product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ PDI / P4HB Antibody (aa499-509) LS-B3137
catalog :
LS-B3137
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
reactivity :
human, rat, domestic rabbit
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 2
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; domestic rabbit; 1:200; fig 7
Steplewski A, Fertala J, Beredjiklian P, Abboud J, Wang M, Namdari S, et al. Auxiliary proteins that facilitate formation of collagen-rich deposits in the posterior knee capsule in a rabbit-based joint contracture model. J Orthop Res. 2016;34:489-501 pubmed publisher
  • immunocytochemistry; rat
  • immunohistochemistry; rat; 1:5000; loading ...; fig 4
  • immunocytochemistry; human
Wong K, Sun F, Trudel G, Sebastiani P, Laneuville O. Temporal gene expression profiling of the rat knee joint capsule during immobilization-induced joint contractures. BMC Musculoskelet Disord. 2015;16:125 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B3137 image 1
Anti-P4HB antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
product information
AntibodyID :
97948
AntibodyName :
LS-B3137
TargetSpecies :
Rat
Host Species :
Mouse
Product Name :
IHC-plus™ PDI / P4HB Antibody (aa499-509) LS-B3137
Specificity :
Recognizes rat protein disulfide isomerase at ~58kD.
ClonalityDesc :
Monoclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa499-509
PresentationDesc :
PBS, pH 7.2, 0.09% Sodium Azide, 50% Glycerol
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Synthetic peptide corresponding to aa499-509 of rat protein disulfide isomerase (KLH coupled).
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Aliquots are stable for 1 year after receipt.
IsotypeName :
IgG1
Gene :
PDI / P4HB
StandardGeneSymbol :
P4HB
Reactivity :
Xenopus, Chicken, Sheep, Bovine, Rabbit, Mouse, Dog, Guinea pig, Rat, Hamster, Pig, Human, Monkey
Usage :
ICC, IHC, IHC-P (5 µg/ml), WB
ShortWebDescription :
P4HB antibody LS-B3137 is an unconjugated mouse monoclonal antibody to P4HB (PDI) (aa499-509) from rat. It is reactive with human, mouse, rat and other species. Validated for ICC, IHC and WB. Cited in 1 publication.
UsageText :
Immunohistochemistry: LS-B3137 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3137 was determined to be 5 ug/ml. Positive control: HeLa Heat Shocked Cell Lysate, MCF-7 cell lysate.
Synonyms :
P4HB, ERBA2L, DSI, GIT, p4HB, p4Hbeta, PDIA1, PHDB, PO4DB, PROHB, Protocollagen hydroxylase, Protein disulfide-isomerase, PO4HB, PDI
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.