product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ Basigin / Emmprin / CD147 Antibody (clone MEM-M6/1) LS-B3133
catalog :
LS-B3133
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
MEM-M6/1
reactivity :
human
application :
western blot, immunohistochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; human; 1:300; fig 5f
Zhu D, Wang Z, Zhao J, Calimeri T, Meng J, Hideshima T, et al. The Cyclophilin A-CD147 complex promotes the proliferation and homing of multiple myeloma cells. Nat Med. 2015;21:572-80 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B3133 image 1
Anti-CD147 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
product information
AntibodyID :
97944
AntibodyName :
LS-B3133
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IHC-plus™ Basigin / Emmprin / CD147 Antibody (clone MEM-M6/1) LS-B3133
ClonalityDesc :
Monoclonal
CloneName :
MEM-M6/1
AntibodyModification :
Unconjugated
PresentationDesc :
PBS, 0.09% Sodium Azide
ImmunogenType :
Recombinant protein
ImmunogenDesc :
Recombinant CD147Rg consisting of the cDNA coding for the hinge region CH2 and CH3 domain of human IgG1.
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
IsotypeName :
IgG1
Gene :
Basigin / Emmprin / CD147
StandardGeneSymbol :
BSG
Reactivity :
Human
Usage :
Flo (1:50 - 1:100), IHC, IHC-P (10 µg/ml), IP, WB
ShortWebDescription :
CD147 antibody LS-B3133 is an unconjugated mouse monoclonal antibody to human CD147 (Basigin / Emmprin). Validated for Flow, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
UsageText :
Immunohistochemistry: LS-B3133 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3133 was determined to be 10 ug/ml. Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells or 100 ul whole blood.
Synonyms :
BSG, 5F7, Basigin, Basigin (Ok blood group), CD147 antigen, EMMPRIN, OK, OK blood group antigen, TCSF, CD147, M6
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.