product summary
request information :
company name :
LifeSpan Biosciences
product type :
antibody
product name :
Anti-Calreticulin
catalog :
LS-B3131
quantity :
price :
clonality :
polyclonal
host :
chicken
reactivity :
human, mouse
application :
immunohistochemistry - paraffin section, western blot, immunoprecipitation, immunohistochemistry, immunocytochemistry
more info or order :
LifeSpan Biosciences product webpage
image
image 1 :
LifeSpan Biosciences LS-B3131 image 1
Human Brain, Cerebellum (formalin-fixed, paraffin-embedded) stained with CALR antibody LS-B3131 at 2.5 ug/ml followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
image 2 :
LifeSpan Biosciences LS-B3131 image 2
Human Prostate (formalin-fixed, paraffin-embedded) stained with CALR antibody LS-B3131 at 2.5 ug/ml followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
product information
?AntibodyID :
97942
AntibodyName :
LS-B3131
TargetSpecies :
Mouse
Host Species :
Chicken
Product Name :
Anti-Calreticulin
Specificity :
Recognizes mouse Calreticulin. Species cross-reactivity: human, rabbit, rat, canine and hamster.
ClonalityDesc :
Polyclonal
AntigenModification :
aa399-414
PresentationDesc :
Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Synthetic peptide: DEKEEDEEESPGQAKD, corresponding to amino acids 399-414 of mouse Calreticulin. Percent identity by BLAST analysis: Mouse (100%); Rat (81%).
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Short term: 4°C. Long term: Aliquot and add glycerol (40-50%). Store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgY
Gene :
Calreticulin
StandardGeneSymbol :
CALR
Reactivity :
Human, Mouse
Usage :
ICC, IF, IHC-P (2.5 µg/ml), IP, WB (1:2000)
UsageText :
Immunohistochemistry: LS-B3131 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3131 was determined to be 2.5 ug/ml. Western Blot: 1:2000. Detects an ~60kD protein representing Calreticulin and an ~120kD unidentified protein from rat brain extract.
Synonyms :
CALR, Calregulin, CC1qR, CRP55, CRT, CRTC, ERp60, Grp60, RO, SSA, Calreticulin, HACBP
SwissProtProtoID :
P27797
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
antibodies@lsbio.com
www.lsbio.com
1-206-464-1554
headquarters: usa
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.