IHC-plus™ PLIN2 / ADFP / Adipophilin Antibody (aa150-250) LS-B3121 | LifeSpan Biosciences LS-B3121 product information

product summary

company name :

LifeSpan Biosciences

product type :

antibody

product name :

IHC-plus™ PLIN2 / ADFP / Adipophilin Antibody (aa150-250) LS-B3121

catalog :

LS-B3121

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

LifeSpan Biosciences product webpage

citations: 6

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; human	De Paepe M, Mao Q, Chu S, Zhang Y, Luks F. Clinicoplacental correlates of amniocyte vacuolization in association with gastroschisis. Placenta. 2017;57:87-93 pubmed publisher
immunocytochemistry; human western blot; human immunocytochemistry; mouse western blot; mouse	Li Z, Schulze R, Weller S, Krueger E, Schott M, Zhang X, et al. A novel Rab10-EHBP1-EHD2 complex essential for the autophagic engulfment of lipid droplets. Sci Adv. 2016;2:e1601470 pubmed publisher
	Mardani I, Tomas Dalen K, Drevinge C, Miljanovic A, Ståhlman M, Klevstig M, et al. Plin2-deficiency reduces lipophagy and results in increased lipid accumulation in the heart. Sci Rep. 2019;9:6909 pubmed publisher
	Nardi F, Franco O, Fitchev P, Morales A, Vickman R, Hayward S, et al. DGAT1 Inhibitor Suppresses Prostate Tumor Growth and Migration by Regulating Intracellular Lipids and Non-Centrosomal MTOC Protein GM130. Sci Rep. 2019;9:3035 pubmed publisher
	Janardhan K, Jensen H, Clayton N, Herbert R. Immunohistochemistry in Investigative and Toxicologic Pathology. Toxicol Pathol. 2018;46:488-510 pubmed publisher
	Amrutkar M, Chursa U, Kern M, Nuñez Durán E, Ståhlman M, Sütt S, et al. STK25 is a critical determinant in nonalcoholic steatohepatitis. FASEB J. 2016;30:3628-3643 pubmed

image

image 1 :

Anti-Adipophilin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

product information

AntibodyID :

97932

AntibodyName :

LS-B3121

TargetSpecies :

Human

Host Species :

Rabbit

Product Name :

IHC-plus™ PLIN2 / ADFP / Adipophilin Antibody (aa150-250) LS-B3121

Specificity :

ADFP.

ClonalityDesc :

Polyclonal

AntibodyModification :

Unconjugated

AntigenModification :

aa150-250

PresentationDesc :

PBS, 0.05% Sodium Azide, 30% Glycerol

ImmunogenType :

Synthetic peptide

ImmunogenDesc :

A synthetic peptide made to an internal portion of the human protein, within residues 150-250. [Swiss-Prot# Q99541]. Percent identity by BLAST analysis: Sheep (94%); Rat (84%).

PurificationDesc :

Immunoaffinity purified

RecommendedStorageDesc :

Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

Gene :

PLIN2 / ADFP / Adipophilin

StandardGeneSymbol :

PLIN2

Reactivity :

Human

Usage :

IHC, IHC-P (5 µg/ml), WB (2 µg/ml)

ShortWebDescription :

Adipophilin antibody LS-B3121 is an unconjugated rabbit polyclonal antibody to human Adipophilin (PLIN2 / ADFP) (aa150-250). Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 8 publications.

UsageText :

Immunohistochemistry: LS-B3121 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3121 was determined to be 5 ug/ml.Recommended blocking buffer for Western blot is 5% nonfat dry milk in TBST (0.1% tween-20).

Synonyms :

PLIN2, Adipophilin, ADFP, ADRP, Perilipin 2, Perilipin-2, Adipose differentiation-related protein

SalesRegion :

Worldwide

more info or order :

LifeSpan Biosciences product webpage