product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ CENPF / CENP-F Antibody (aa1759-2093, clone 14C10 1D8) LS-B3046
catalog :
LS-B3046
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
14C10 1D8
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
LifeSpan Biosciences product webpage
citations: 2
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; human; loading ...; fig 3d
Bakr A, Köcher S, Volquardsen J, Petersen C, Borgmann K, Dikomey E, et al. Impaired 53BP1/RIF1 DSB mediated end-protection stimulates CtIP-dependent end resection and switches the repair to PARP1-dependent end joining in G1. Oncotarget. 2016;7:57679-57693 pubmed publisher
Mansour W, Tennstedt P, Volquardsen J, Oing C, Kluth M, Hube Magg C, et al. Loss of PTEN-assisted G2/M checkpoint impedes homologous recombination repair and enhances radio-curability and PARP inhibitor treatment response in prostate cancer. Sci Rep. 2018;8:3947 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B3046 image 1
Anti-CENPF antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
product information
AntibodyID :
97428
AntibodyName :
LS-B3046
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IHC-plus™ CENPF / CENP-F Antibody (aa1759-2093, clone 14C10 1D8) LS-B3046
Specificity :
Reacts with human Mitosin.
ClonalityDesc :
Monoclonal
CloneName :
14C10 1D8
AntibodyModification :
Unconjugated
AntigenModification :
aa1759-2093
PresentationDesc :
PBS
ImmunogenType :
Recombinant protein
ImmunogenDesc :
Protein fragment expressed in E. coli corresponding to amino acids 1759-2093.
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
Keep as concentrated solution. For short term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
IsotypeName :
IgG2a
Gene :
CENPF / CENP-F
StandardGeneSymbol :
CENPF
Reactivity :
Human
Usage :
IF, IHC, IHC-Fr, IHC-P (10 µg/ml), IP, WB (1:500)
ShortWebDescription :
CENP-F antibody LS-B3046 is an unconjugated mouse monoclonal antibody to human CENP-F (CENPF) (aa1759-2093). Validated for IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
UsageText :
Immunohistochemistry: LS-B3046 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3046 was determined to be 10 ug/ml.
Synonyms :
CENPF, AH antigen, Centromere protein F, CENF, CENP-F, Kinetochore protein CENPF, Mitosin, CENP-F kinetochore protein, Hcp-1, PRO1779
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.