This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
PathPlus™ GAD1 / GAD67 Antibody (aa526-537) LS-B3027
catalog :
LS-B3027
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunohistochemistry - paraffin section
product information
AntibodyID :
97408
AntibodyName :
LS-B3027
TargetSpecies :
Human
Host Species :
Goat
Product Name :
PathPlus™ GAD1 / GAD67 Antibody (aa526-537) LS-B3027
Specificity :
Human GAD1 / GAD67. This antibody is expected to recognize isoform GAD67. There is no cross-reactivity expected with GAD2.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa526-537
PresentationDesc :
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
ImmunogenDesc :
Peptide with sequence C-PDSPQRREKLHK, from the internal region of the protein sequence according to NP_000808.2.
PurificationDesc :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
RecommendedStorageDesc :
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Gene :
GAD1 / GAD67
BlockingPeptide :
LS-E26954
StandardGeneSymbol :
GAD1
Reactivity :
Mouse, Human
Usage :
IHC, IHC-P (4 µg/ml), Peptide-ELISA (1:32000), WB (0.5 - 1.5 µg/ml)
ShortWebDescription :
GAD67 antibody LS-B3027 is an unconjugated goat polyclonal antibody to GAD67 (GAD1) (aa526-537) from human. It is reactive with human and mouse. Validated for IHC, Peptide-ELISA and WB.
UsageText :
Immunohistochemistry: LS-B3027 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3027 was determined to be 4 ug/ml. Peptide ELISA: antibody detection limit dilution 1:8000. Western Blot: Approx. 70kD band observed in Human Brain (Cerebellum) and in Mouse and Rat Brain lysates (calculated MW of 66.9kD according to Human NP_000808.2 and 66.6kD according to Mouse NP_032103.2). Recommended concentration: 0.3-1 ug/ml.
Synonyms :
GAD1, CPSQ1, GAD-67, Glutamate decarboxylase 1, GAD67, GAD, SCP
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.