This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ SCNN1G / ENaC Gamma Antibody (aa637-648) LS-B3024
catalog :
LS-B3024
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human
application :
immunohistochemistry, immunohistochemistry - paraffin section
product information
AntibodyID :
97401
AntibodyName :
LS-B3024
TargetSpecies :
Human
Host Species :
Goat
Product Name :
IHC-plus™ SCNN1G / ENaC Gamma Antibody (aa637-648) LS-B3024
Specificity :
Human SCNN1G.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa637-648
PresentationDesc :
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
ImmunogenDesc :
Peptide with sequence C-SNQLTDTQMLDE, from the C-Terminus of protein sequence according to NP_001030.2.
PurificationDesc :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
RecommendedStorageDesc :
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Gene :
SCNN1G / ENaC Gamma
BlockingPeptide :
LS-E26895
StandardGeneSymbol :
SCNN1G
gene family :
Ion Channel
Subfamily :
Sodium channel - amiloride-sensitive
Reactivity :
Human
Usage :
IHC, IHC-P (4 µg/ml), Peptide-ELISA (1:16000)
ShortWebDescription :
ENaC Gamma antibody LS-B3024 is an unconjugated goat polyclonal antibody to human ENaC Gamma (SCNN1G) (aa637-648). Validated for IHC and Peptide-ELISA. Tested on 20 paraffin-embedded human tissues.
UsageText :
Immunohistochemistry: LS-B3024 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3024 was determined to be 4 ug/ml. Peptide ELISA: antibody detection limit dilution 1:16000. Western Blot: Preliminary experiments gave an approx 140 kD band in Human Breast cancer and Colon lysates after 1 ug/ml antibody staining. We cannot find an explanation in the literature for the band we observe given the calculated size of 74.3kD according to NP_001030.2. The 140kD band was successfully blocked by incubation with the immunizing peptide.
Synonyms :
SCNN1G, BESC3, ENaC gamma, ENaCgamma, Gamma-NaCH, Gamma hENaC, HENaC, PHA1, RENaC, SCNEG, ENaC gamma subunit, ENaCg, Gamma-ENaC
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.
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