IHC-plus™ CYP19 / Aromatase Antibody (aa475-486) LS-B3021 | LifeSpan Biosciences LS-B3021 product information

product summary

company name :

LifeSpan Biosciences

product type :

antibody

product name :

IHC-plus™ CYP19 / Aromatase Antibody (aa475-486) LS-B3021

catalog :

LS-B3021

clonality :

polyclonal

host :

domestic goat

conjugate :

nonconjugated

reactivity :

human, rat

application :

western blot, immunohistochemistry, immunohistochemistry - paraffin section

more info or order :

LifeSpan Biosciences product webpage

citations: 1

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; rat	Zhong Y, Dhawan J, Kovoor J, Sullivan J, Zhang W, Choi D, et al. Aromatase and neuroinflammation in rat focal brain ischemia. J Steroid Biochem Mol Biol. 2017;174:225-233 pubmed publisher

image

image 1 :

CYP19 / Aromatase antibody (0.1µg/ml) staining of Human Placenta lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

image 2 :

Anti-Aromatase antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 4 ug/ml.

product information

AntibodyID :

97398

AntibodyName :

LS-B3021

TargetSpecies :

Human

Host Species :

Goat

Product Name :

IHC-plus™ CYP19 / Aromatase Antibody (aa475-486) LS-B3021

Specificity :

Human CYP19A1 / Aromatase. This antibody is expected to recognise both reported isoforms (NP_000094.2 and NP_112503.1), which are identical

ClonalityDesc :

Polyclonal

AntibodyModification :

Unconjugated

AntigenModification :

aa475-486

PresentationDesc :

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA

ImmunogenDesc :

Peptide with sequence C-HDLSLHPDETKN, from the internal region of the protein sequence according to NP_000094.2NP_112503.1.

PurificationDesc :

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

RecommendedStorageDesc :

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Gene :

CYP19 / Aromatase

BlockingPeptide :

LS-E26615

StandardGeneSymbol :

CYP19A1

gene family :

Cytochrome P450

Reactivity :

Human

Usage :

IHC, IHC-P (4 µg/ml), Peptide-ELISA (1:128000), WB (0.1 - 0.3 µg/ml)

ShortWebDescription :

Aromatase antibody LS-B3021 is an unconjugated goat polyclonal antibody to human Aromatase (CYP19) (aa475-486). Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.

UsageText :

Immunohistochemistry: LS-B3021 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3021 was determined to be 4 ug/ml.

Synonyms :

CYP19A1, ARO1, CYAR, CPV1, CYPXIX, Cytochrome P-450AROM, Cytochrome P450 19A1, CYP19, Estrogen synthase, Estrogen synthetase, P-450AROM, ARO, Aromatase

SalesRegion :

Worldwide

more info or order :

LifeSpan Biosciences product webpage

company information

LifeSpan Biosciences

2401 Fourth Avenue, Suite 900
Seattle, WA 98121

CustomerSupport@lsbio.com

https://www.lsbio.com

1-206-464-1554

headquarters: USA

Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.