product summary
request information :
company name :
LifeSpan Biosciences
product type :
antibody
product name :
Anti-RARA / RAR Alpha
catalog :
LS-B3004
quantity :
price :
clonality :
polyclonal
host :
goat
reactivity :
human, mouse
application :
immunohistochemistry - paraffin section, western blot, ELISA, immunohistochemistry
more info or order :
LifeSpan Biosciences product webpage
image
image 1 :
LifeSpan Biosciences LS-B3004 image 1
Human Brain, Cerebellum (formalin-fixed, paraffin-embedded) stained with RARA antibody LS-B3004 at 4 ug/ml followed by biotinylated anti-goat IgG secondary antibody LS-D3, alkaline phosphatase-streptavidin and chromogen.
product information
?AntibodyID :
97381
AntibodyName :
LS-B3004
TargetSpecies :
Human
Host Species :
Goat
Product Name :
Anti-RARA / RAR Alpha
Specificity :
Epitope is from the mouse/rat sequence. Human sequence is SPSLSPSSNRSSPATHSP and thus highly similar.
ClonalityDesc :
Polyclonal
AntigenModification :
C-Terminus
PresentationDesc :
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Synthetic peptide C-SPSLSPSSHRSSPATQSP from the C-terminus of human RARA (NP_000955.1; NP_001138773.1; NP_001019980.1; NP_001138774.1). Percent identity by BLAST analysis: Mouse, Rat (100%); Human, Monkey, Marmoset, Elephant, Panda, Bovine, Dog, Horse, Pig, Opossum, Chicken, Seabass (89%); Pufferfish (83%).
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Store at -20°C. Minimize freezing and thawing.
Gene :
RARA / RAR Alpha
StandardGeneSymbol :
RARA
gene family :
NHR
Subfamily :
NR1 Thyroid hormone-like
Reactivity :
Human, Mouse, Rat
Usage :
ELISA (1:128000), IHC-P (4 µg/ml), WB (0.03 - 0.3 µg/ml)
UsageText :
Although raised against mouse the antibody has been tested with human samples in Western blot and IHC.Immunohistochemistry: LS-B3004 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3004 was determined to be 4 ug/ml.
Synonyms :
RARA, NR1B1, PML-RAR, RAR-alpha, RARalpha, Retinoic acid receptor alpha, Pml-rara, RAR, RAR alpha, Retinoic acid receptor, alpha
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
antibodies@lsbio.com
www.lsbio.com
1-206-464-1554
headquarters: usa
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.