product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ SDC2 / Syndecan 2 Antibody (Internal) LS-B2981
catalog :
LS-B2981
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 2
Published Application/Species/Sample/DilutionReference
  • western blot; human
Waters A, Stafman L, Garner E, Mruthyunjayappa S, Stewart J, Friedman G, et al. Effect of Repeat Dosing of Engineered Oncolytic Herpes Simplex Virus on Preclinical Models of Rhabdomyosarcoma. Transl Oncol. 2016;9:419-430 pubmed publisher
Corti F, Wang Y, Rhodes J, Atri D, Archer Hartmann S, Zhang J, et al. N-terminal syndecan-2 domain selectively enhances 6-O heparan sulfate chains sulfation and promotes VEGFA165-dependent neovascularization. Nat Commun. 2019;10:1562 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B2981 image 1
Anti-SDC2 / Syndecan 2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
product information
AntibodyID :
96655
AntibodyName :
LS-B2981
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ SDC2 / Syndecan 2 Antibody (Internal) LS-B2981
Specificity :
Recognizes human syndecan-2 (fibroglycan). It recognizes the unglyconated form and may recognize the glyconated form. On Western Blots, it identifies a single band at ~22kD. An additional band at ~40kD may also be observed which may represent a glycanated form. Reactivity has been confirmed with human A-549 lung carcinoma, HEP-G2 hepatocellular carcinoma, HT-29 colorectal adenocarcinoma, K-562 chronic myelogenous leukemia-bone marrow, TF-1 erythroleukemia-bone marrow and U-2 OS osteosarcoma cell lysates.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
Internal
PresentationDesc :
PBS, pH 7.4, 0.09% Sodium Azide
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Synthetic peptide derived from the internal region of human syndecan-2 (fibroglycan).
PurificationDesc :
Affinity purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG
Gene :
SDC2 / Syndecan 2
StandardGeneSymbol :
SDC2
Reactivity :
Human
Usage :
ELISA (0.1 - 1 µg/ml), IHC, IHC-P (5 µg/ml), WB (1 - 3 µg/ml)
ShortWebDescription :
Syndecan 2 antibody LS-B2981 is an unconjugated rabbit polyclonal antibody to human Syndecan 2 (SDC2) (Internal). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 3 publications.
UsageText :
Immunohistochemistry: LS-B2981 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2981 was determined to be 5 ug/ml.
Synonyms :
SDC2, CD362, CD362 antigen, HSPG, HSPG1, Fibroglycan, Syndecan proteoglycan 2, SYND2, Syndecan 2, Syndecan-2
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.