product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ IRF7 Antibody (aa1-150, clone 3D9) LS-B2945
catalog :
LS-B2945
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
3D9
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; human
Restivo G, Nguyen B, Dziunycz P, Ristorcelli E, Ryan R, Özuysal Ö, et al. IRF6 is a mediator of Notch pro-differentiation and tumour suppressive function in keratinocytes. EMBO J. 2011;30:4571-85 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B2945 image 1
Anti-IRF7 antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.
image 2 :
LifeSpan Biosciences LS-B2945 image 2
Cell lysates of Jurkat (40 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human IRF7 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
product information
AntibodyID :
96619
AntibodyName :
LS-B2945
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IHC-plus™ IRF7 Antibody (aa1-150, clone 3D9) LS-B2945
ClonalityDesc :
Monoclonal
CloneName :
3D9
AntibodyModification :
Unconjugated
AntigenModification :
aa1-150
PresentationDesc :
PBS, pH 7.4, 0.02% Sodium Azide, 10% Glycerol
ImmunogenType :
Recombinant protein
ImmunogenDesc :
Recombinant human IRF7 (1-150aa) purified from E. coli.
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG1,k
Gene :
IRF7
StandardGeneSymbol :
IRF7
Reactivity :
Human
Usage :
ELISA, IHC, IHC-P (20 µg/ml), WB (1:1000 - 1:2000)
ShortWebDescription :
IRF7 antibody LS-B2945 is an unconjugated mouse monoclonal antibody to human IRF7 (aa1-150). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
UsageText :
Immunohistochemistry: LS-B2945 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2945 was determined to be 20 ug/ml.
Synonyms :
IRF7, Interferon regulatory factor 7, IRF-7, IRF7A, IRF-7H
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.