product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ NT5E / eNT / CD73 Antibody (aa27-252, clone 2B6) LS-B2943
catalog :
LS-B2943
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
2B6
reactivity :
human, mouse
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; mouse
Iriyama T, Sun K, Parchim N, Li J, Zhao C, Song A, et al. Elevated placental adenosine signaling contributes to the pathogenesis of preeclampsia. Circulation. 2015;131:730-41 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B2943 image 1
Anti-NT5E / CD73 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
image 2 :
LifeSpan Biosciences LS-B2943 image 2
Cell lysates of human mesenchymal stem cell (30 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human CD73 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL.
image 3 :
LifeSpan Biosciences LS-B2943 image 3
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CD73 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HepG2 cell lysate Lane 2.: WiDr cell lysate Lane 3.: A431 cell lysate
product information
AntibodyID :
96617
AntibodyName :
LS-B2943
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IHC-plus™ NT5E / eNT / CD73 Antibody (aa27-252, clone 2B6) LS-B2943
ClonalityDesc :
Monoclonal
CloneName :
2B6
AntibodyModification :
Unconjugated
AntigenModification :
aa27-252
PresentationDesc :
PBS, pH 7.4, 0.1% Sodium Azide
ImmunogenType :
Recombinant protein
ImmunogenDesc :
Recombinant human CD73 (27-252aa) purified from E. coli.
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG2b,k
Gene :
NT5E / eNT / CD73
StandardGeneSymbol :
NT5E
Reactivity :
Human
Usage :
ELISA, IF, IHC, IHC-P (10 µg/ml), SDS-PAGE, WB (1:1000 - 1:2000)
ShortWebDescription :
CD73 antibody LS-B2943 is an unconjugated mouse monoclonal antibody to human CD73 (NT5E / eNT) (aa27-252). Validated for ELISA, IF, IHC, SDS-PAGE and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
UsageText :
Immunohistochemistry: LS-B2943 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2943 was determined to be 10 ug/ml.
Synonyms :
NT5E, 5-nucleotidase, ecto (CD73), 5'-nucleotidase, 5 nucleotidase (CD73), 5-NT, 5-nucleotidase, 5'-nucleotidase, ecto (CD73), CD73, CD73 antigen, E5NT, Ecto-5-nucleotidase, ENT, EN, NT, Purine 5-Prime-Nucleotidase, NT5, NTE
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.