product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ PSPH Antibody (aa1-225, clone 3G12) LS-B2935
catalog :
LS-B2935
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
3G12
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section
citations: 1
Reference
Seo H, Bazer F, Burghardt R, Johnson G. Immunohistochemical Examination of Trophoblast Syncytialization during Early Placentation in Sheep. Int J Mol Sci. 2019;20: pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B2935 image 1
Anti-PSPH antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
image 2 :
LifeSpan Biosciences LS-B2935 image 2
Cell lysates of Mouse brain (30 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PSP (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
product information
AntibodyID :
96609
AntibodyName :
LS-B2935
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IHC-plus™ PSPH Antibody (aa1-225, clone 3G12) LS-B2935
ClonalityDesc :
Monoclonal
CloneName :
3G12
AntibodyModification :
Unconjugated
AntigenModification :
aa1-225
PresentationDesc :
PBS, pH 7.4, 0.02% sodium azide, 10% glycerol.
ImmunogenType :
Recombinant protein
ImmunogenDesc :
Recombinant human PSP (1-225aa) purified from E. coli.
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
Short term: store at 2°C to 8°C for up to 1 week. Long term: aliquot and store at -20°C to -80°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG1,k
Gene :
PSPH
StandardGeneSymbol :
PSPH
Reactivity :
Mouse, Human
Usage :
ELISA, IHC, IHC-P (10 µg/ml), WB (1:1000 - 1:2000)
ShortWebDescription :
PSPH antibody LS-B2935 is an unconjugated mouse monoclonal antibody to PSPH (aa1-225) from human. It is reactive with human and mouse. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
UsageText :
Immunohistochemistry: LS-B2935 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2935 was determined to be 10 ug/ml.
Synonyms :
PSPH, L-3-phosphoserine phosphatase, Phosphoserine phosphatase, PSPase, PSP, PSPHD
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.