This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
PathPlus™ IGF1R / IGF1 Receptor Antibody (aa1264-1367, clone JBW902) LS-B2905
catalog :
LS-B2905
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
JBW902
reactivity :
human
application :
western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section
product information
AntibodyID :
96579
AntibodyName :
LS-B2905
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
PathPlus™ IGF1R / IGF1 Receptor Antibody (aa1264-1367, clone JBW902) LS-B2905
Specificity :
Recognizes human IGF-1 receptor beta chain, MW 95kD. Recognizes transmembrane domain. Not expected to cross-react with INSR based on sequence homology.
ClonalityDesc :
Monoclonal
CloneName :
JBW902
AntibodyModification :
Unconjugated
AntigenModification :
aa1264-1367
PresentationDesc :
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide
ImmunogenType :
Fusion protein
ImmunogenDesc :
GST fusion protein corresponding to residues 1264-1367 of human IGF-1 Receptor.
PurificationDesc :
Protein A purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG2a
Gene :
IGF1R / IGF1 Receptor
StandardGeneSymbol :
IGF1R
gene family :
Protein Kinase
Subfamily :
Insulin receptor
Reactivity :
Mouse, Human
Usage :
IHC, IHC-P (5 µg/ml), IP, WB
ShortWebDescription :
IGF1 Receptor antibody LS-B2905 is an unconjugated mouse monoclonal antibody to IGF1 Receptor (IGF1R) (aa1264-1367) from human. It is reactive with human and mouse. Validated for IHC, IP and WB.
UsageText :
Immunohistochemistry: LS-B2905 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2905 was determined to be 5 ug/ml. Western Blot: 0.1-2 ug/ml. Detects the beta chain of IGF1R in RIPA lysates from C4 cells (mouse 3T3 cells that have been transfected with the human IGF1R) and in RIPA lysates from mouse 3T3/A31 cells. C4 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with LS-B2905. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 4 ug/ml immunoprecipitates IGF1R from 1 mg of RIPA lysates from C4 cells (mouse 3T3 cells that have been transfected with the human IGF1R) and in RIPA lysates from mouse 3T3/A31 cells.
Synonyms :
IGF1R, CD221 antigen, CD221, IGF1 Receptor, IGFR, IGF-I receptor, Soluble IGF1R variant 1, Soluble IGF1R variant 2, Type 1 IGF receptor, IGFIR, JTK13
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.