IHC-plus™ ITGB1 / Integrin Beta 1 / CD29 Antibody (clone HUTS-4) LS-B2861 | LifeSpan Biosciences LS-B2861 product information

product summary

company name :

LifeSpan Biosciences

product type :

antibody

product name :

IHC-plus™ ITGB1 / Integrin Beta 1 / CD29 Antibody (clone HUTS-4) LS-B2861

catalog :

LS-B2861

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

HUTS-4

reactivity :

human, rat

application :

western blot, ELISA, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

more info or order :

LifeSpan Biosciences product webpage

citations: 3

Published Application/Species/Sample/Dilution	Reference
western blot; human	Hatanaka K, Ito K, Shindo T, Kagaya Y, Ogata T, Eguchi K, et al. Molecular mechanisms of the angiogenic effects of low-energy shock wave therapy: roles of mechanotransduction. Am J Physiol Cell Physiol. 2016;311:C378-85 pubmed publisher
immunohistochemistry - paraffin section; human; 1:100; loading ...; fig 6i	Romagnoli M, Mineva N, Polmear M, Conrad C, Srinivasan S, Loussouarn D, et al. ADAM8 expression in invasive breast cancer promotes tumor dissemination and metastasis. EMBO Mol Med. 2014;6:278-94 pubmed publisher
western blot; rat	Du J, Chen X, Liang X, Zhang G, Xu J, He L, et al. Integrin activation and internalization on soft ECM as a mechanism of induction of stem cell differentiation by ECM elasticity. Proc Natl Acad Sci U S A. 2011;108:9466-71 pubmed publisher

image

image 1 :

Anti-Integrin Beta 1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

product information

AntibodyID :

96535

AntibodyName :

LS-B2861

TargetSpecies :

Human

Host Species :

Mouse

Product Name :

IHC-plus™ ITGB1 / Integrin Beta 1 / CD29 Antibody (clone HUTS-4) LS-B2861

Specificity :

Specific for active conformation of human b1 integrins. Does not augment the affinity of beta 1 for ligands. Species cross-reactivity: rat.

ClonalityDesc :

Monoclonal

CloneName :

HUTS-4

AntibodyModification :

Unconjugated

PresentationDesc :

PBS, pH 7.1. No preservatives.

ImmunogenType :

Cells

ImmunogenDesc :

Human b1 integrins purified from Triton X-100 lysates of human lung, liver, and skeletal muscle tissues. Specifically selected due to its increased reactivity with T-lymphoblasts upon Mn2+ stimulation.

PurificationDesc :

Purified

RecommendedStorageDesc :

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

IsotypeName :

IgG2b

Gene :

ITGB1 / Integrin Beta 1 / CD29

StandardGeneSymbol :

ITGB1

gene family :

Integrin

Reactivity :

Rat, Human

Usage :

ELISA, Flo, IHC, IHC-P (10 µg/ml), IHC-Pfa, WB

ShortWebDescription :

CD29 antibody LS-B2861 is an unconjugated mouse monoclonal antibody to CD29 (ITGB1 / Integrin Beta 1) from human. It is reactive with human and rat. Validated for ELISA, Flow, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.

UsageText :

Immunohistochemistry: LS-B2861 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2861 was determined to be 10 ug/ml. FACS: 2.5 ug/5x10^5 cells.

Synonyms :

ITGB1, CD29, CD29 antigen, Integrin beta-1, MDF2, MSK12, Integrin VLA-4 beta subunit, VLA-BETA, VLAB, Beta 1 integrin, FNRB, GPIIA, Integrin Beta 1, VLA-4 subunit beta

SalesRegion :

Worldwide

more info or order :

LifeSpan Biosciences product webpage

company information

LifeSpan Biosciences

2401 Fourth Avenue, Suite 900
Seattle, WA 98121

CustomerSupport@lsbio.com

https://www.lsbio.com

1-206-464-1554

headquarters: USA

Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.