This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ ACTA2 / Smooth Muscle Actin Antibody (N-Terminus, Azide-free) LS-B2847
catalog :
LS-B2847
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
reactivity :
human
application :
immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
product information
AntibodyID :
96521
AntibodyName :
LS-B2847
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IHC-plus™ ACTA2 / Smooth Muscle Actin Antibody (N-Terminus, Azide-free) LS-B2847
Specificity :
Recognizes the alpha-smooth muscle isoform of actin. Does not cross-react with actin from fibroblasts (b-and g-cytoplasmic), striated muscle (a-sarcomeric), and myocardium (a-myocardial). Species cross-reactivity: Human, baboon, bovine, rabbit, mouse, rat and chicken.
ClonalityDesc :
Monoclonal
AntibodyModification :
Azide-free Unconjugated
AntigenModification :
N-Terminus
PresentationDesc :
PBS, pH 7.4. No preservatives added. No stabilizing proteins added. Glycerol free.
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
N-terminal decapeptide of the alpha smooth muscle isoform of actin; acetylated at the N-terminus. Epitope Location: Acetyl group and the first 4 amino acids on the N-terminal end of the peptide chain of alpha-smooth actin. Cellular Localization: Cytoplasm.
PurificationDesc :
Protein A purified
RecommendedStorageDesc :
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG2a,k
Gene :
ACTA2 / Smooth Muscle Actin
StandardGeneSymbol :
ACTA2
Reactivity :
Chicken, Bovine, Rabbit, Mouse, Rat, Human
Usage :
IF, IHC, IHC-Fr, IHC-P (5 µg/ml)
ShortWebDescription :
Smooth Muscle Actin antibody LS-B2847 is an unconjugated mouse monoclonal antibody to Smooth Muscle Actin (ACTA2) (N-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for IF and IHC. Tested on 20 paraffin-embedded human tissues.
UsageText :
Immunohistochemistry: LS-B2847 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2847 was determined to be 5 ug/ml. Positive control: Smooth muscle or leiomyosarcoma. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
Synonyms :
ACTA2, ACTVS, Alpha-actin-2, Alpha-cardiac actin, AAT6, Actin, aortic smooth muscle, ACTSA, Smooth Muscle Actin, MYMY5, SMA
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.