This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ CXCL1 / GRO Alpha Antibody LS-B2843
catalog :
LS-B2843
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, neutralization, immunohistochemistry - paraffin section
product information
AntibodyID :
96517
AntibodyName :
LS-B2843
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ CXCL1 / GRO Alpha Antibody LS-B2843
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
PresentationDesc :
Lyophilized from PBS, pH 7.2
ImmunogenType :
Recombinant protein
ImmunogenDesc :
Highly pure (>98%) recombinant hGRO/MGSA (human GRO/MGSA)
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
ResuspensionLiquidDesc :
Reconstitute in sterile water to 0.1-1 mg/ml.
Gene :
CXCL1 / GRO Alpha
StandardGeneSymbol :
CXCL1
gene family :
Intercrine
Subfamily :
Intercrine alpha
Reactivity :
Human
Usage :
ELISA (0.2 µg/ml), IHC, IHC-P (5 µg/ml), Neut (1.5 - 3 µg/ml), WB (0.1 - 0.2 µg/ml)
ShortWebDescription :
GRO Alpha antibody LS-B2843 is an unconjugated rabbit polyclonal antibody to human GRO Alpha (CXCL1). Validated for ELISA, IHC, Neut and WB. Tested on 20 paraffin-embedded human tissues.
UsageText :
Immunohistochemistry: LS-B2843 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2843 was determined to be 5 ug/ml. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hGRO/MGSA 100 ng/ml), a concentration of 1.5 - 3.0 ug/ml of this antibody is required. ELISA: To detect hGRO/MGSA by direct ELISA (using 100 ul/well antibody solution) a concentration of at least 0.5 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hGRO/MGSA. Western Blot: To detect hGRO/MGSA by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGRO/MGSA is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Synonyms :
CXCL1, C-X-C motif chemokine 1, Fibroblast secretory protein, FSP, GRO1, GROa, Growth-regulated alpha protein, GRO-alpha(1-73), MGSA, MGSA-a, Small inducible cytokine b1, GRO, MGSA alpha, NAP-3, SCYB1
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.