This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ FGFR / FGF Receptor Antibody (phospho-Tyr653/Tyr654) LS-B2833
catalog :
LS-B2833
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
antigen modification :
phosphorylated
reactivity :
human
application :
western blot, immunohistochemistry, immunohistochemistry - paraffin section
product information
AntibodyID :
90090
AntibodyName :
LS-B2833
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IHC-plus™ FGFR / FGF Receptor Antibody (phospho-Tyr653/Tyr654) LS-B2833
Specificity :
Detects overexpressed levels of Tyr653/654 phosphorylated FGF receptors. Slightly cross-reactivity with activated PDGF and insulin/IGF-I receptors. Does not cross-react with other tyrosine-phosphorylated proteins.
ClonalityDesc :
Monoclonal
AntibodyModification :
Unconjugated
AntigenModification :
pTyr653/Tyr654
PresentationDesc :
10 mM HEPES-Sodium, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 0.02% Sodium Azide, 50% Glycerol
ImmunogenType :
Synthetic phospho-peptide
ImmunogenDesc :
Synthetic phosphopeptide corresponding to residues surrounding Tyr653/654 of human FGF receptor-1 (KLH coupled). The corresponding sequences are identical in FGF receptor-2, -3 and -4.
PurificationDesc :
Ascites
RecommendedStorageDesc :
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG2b
Gene :
FGFR / FGF Receptor
StandardGeneSymbol :
FGFR
Reactivity :
Human
Usage :
IHC, IHC-P (1:50), WB (1:1000)
ShortWebDescription :
FGF Receptor antibody LS-B2833 is an unconjugated mouse monoclonal antibody to human FGF Receptor (FGFR) (pTyr653/Tyr654). Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
UsageText :
Immunohistochemistry: LS-B2833 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2833 was determined to be 1:50. Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4uC with gentle shaking, overnight.
Synonyms :
, FGFR
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.