This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ IL7R / CD127 Antibody (phospho-Tyr449) LS-B2831
catalog :
LS-B2831
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
antigen modification :
phosphorylated
reactivity :
human, mouse
application :
western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section
product information
AntibodyID :
90088
AntibodyName :
LS-B2831
TargetSpecies :
Mouse
Host Species :
Rabbit
Product Name :
IHC-plus™ IL7R / CD127 Antibody (phospho-Tyr449) LS-B2831
Specificity :
This antibody is specific for mouse IL-7 protein phosphorylated at Y449. A BLAST analysis was used to suggest cross-reactivity with IL-7 from human, mouse and rat sources based on 100% homology with the immunizing sequence. Cross-reactivity with IL-7 from other sources has not been determined.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
pTyr449
PresentationDesc :
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues near Y449 of mouse IL-7 protein.
PurificationDesc :
Affinity purified
RecommendedStorageDesc :
Product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
IsotypeName :
IgG
Gene :
IL7R / CD127
StandardGeneSymbol :
IL7R
gene family :
Interleukin
Reactivity :
Mouse, Rat, Human
Usage :
ELISA (1:5000 - 1:50000), IHC, IHC-P (5 µg/ml), WB (1:10000)
ShortWebDescription :
CD127 antibody LS-B2831 is an unconjugated rabbit polyclonal antibody to CD127 (IL7R) (pTyr449) from mouse. It is reactive with human, mouse and rat. Validated for ELISA, IHC and WB.
UsageText :
Immunohistochemistry: LS-B2831 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2831 was determined to be 5 ug/ml. Western Blot: Expect a band approximately 55 kD in size corresponding to phosphorylated IL-7 in the appropriate cell lysate or extract. This phospho-specific polyclonal antibody reacts with mouse IL-7 pY449 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.
Synonyms :
IL7R, CD127, CD127 antigen, IL-7RA, IL-7 receptor subunit alpha, IL7RA, ILRA, Interleukin 7 receptor, CDW127, IL-7R subunit alpha, IL-7R-alpha
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.