product summary
Loading...
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ HIF1A / HIF1 Alpha Antibody (aa432-528) LS-B2823
catalog :
LS-B2823
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Reference
Iriyama T, Wang W, Parchim N, Sayama S, Kumasawa K, Nagamatsu T, et al. Reciprocal upregulation of hypoxia-inducible factor-1α and persistently enhanced placental adenosine signaling contribute to the pathogenesis of preeclampsia. FASEB J. 2020;34:4041-4054 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B2823 image 1
Anti-HIF1A antibody IHC of human kidney, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100. This image was taken for the unconjugated form of this product. Other forms have not been tested.
product information
AntibodyID :
90080
AntibodyName :
LS-B2823
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ HIF1A / HIF1 Alpha Antibody (aa432-528) LS-B2823
Specificity :
HIF-1 alpha.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa432-528
PresentationDesc :
PBS, 0.05% Sodium Azide
ImmunogenType :
Fusion protein
ImmunogenDesc :
Fusion protein containing amino acids 432-528 of human HIF-1 alpha [UniProt# Q16665]
PurificationDesc :
Affinity purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Gene :
HIF1A / HIF1 Alpha
StandardGeneSymbol :
HIF1A
Reactivity :
Xenopus, Zebrafish, Rat, Mouse, Dog, Guinea pig, Human, Primate
Usage :
IHC, IHC-P (1:100), WB
ShortWebDescription :
HIF1 Alpha antibody LS-B2823 is an unconjugated rabbit polyclonal antibody to HIF1 Alpha (HIF1A) (aa432-528) from human. It is reactive with human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
UsageText :
Immunohistochemistry: LS-B2823 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2823 was determined to be 1:100.
Synonyms :
HIF1A, ARNT-interacting protein, ARNT interacting protein, BHLHe78, HIF1 Alpha, Hypoxia-inducible factor1alpha, HIF-1A, HIF1, MOP1, Member of PAS superfamily 1, PASD8, HIF-1-alpha, HIF-1alpha, HIF1-ALPHA, Member of PAS protein 1
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.