product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ c-Met Antibody (Cytoplasmic Domain) LS-B2812
catalog :
LS-B2812
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
reactivity :
human
application :
immunohistochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
LifeSpan Biosciences product webpage
citations: 4
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - paraffin section; human
Varesano S, Salvi S, Boccardo S, Ravetti J, Ferro P, Canessa P, et al. Amplification of MET in a Patient with Malignant Pleural Mesothelioma. J Thorac Oncol. 2015;10:e103-4 pubmed publisher
Salvi S, Varesano S, Boccardo S, Ravetti J, Canessa P, Pistillo M, et al. EGFR Status in Mesothelioma: Possible Implications for the Efficacy of Anti-EGFR and Anti-MET Therapies. J Thorac Oncol. 2016;11:e78-80 pubmed publisher
Varesano S, Salvi S, Boccardo S, Ravetti J, Pistillo M, Canessa P, et al. MET Gene Status in Malignant Mesothelioma Using Fluorescent In Situ Hybridization. J Thorac Oncol. 2016;11:e28-30 pubmed publisher
Fukuizumi A, Akagi K, Sakai H. A Case of Atypical Carcinoid: Harboring Variant 3a/b EML4-ALK Rearrangement. J Thorac Oncol. 2015;10:e104-6 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B2812 image 1
Anti-c-Met antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
product information
AntibodyID :
90067
AntibodyName :
LS-B2812
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IHC-plus™ c-Met Antibody (Cytoplasmic Domain) LS-B2812
Specificity :
Recognizes c-Met.
ClonalityDesc :
Monoclonal
AntibodyModification :
Unconjugated
AntigenModification :
Cytoplasmic Domain
PresentationDesc :
PBS, pH 7.4, 0.09% Sodium Azide, 1% BSA
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Synthetic peptide derived from cytoplasmic domain of human c-Met protein
PurificationDesc :
Purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG2a
Gene :
c-Met
StandardGeneSymbol :
MET
gene family :
Protein Kinase
Subfamily :
HGF Receptor/MET
Reactivity :
Human
Usage :
IHC, IHC-Fr (1:50), IHC-P (10 µg/ml)
ShortWebDescription :
C-Met antibody LS-B2812 is an unconjugated mouse monoclonal antibody to human c-Met (Cytoplasmic Domain). Validated for IHC. Tested on 20 paraffin-embedded human tissues. Cited in 3 publications.
UsageText :
Immunohistochemistry: LS-B2812 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2812 was determined to be 10 ug/ml. Positive control: Breast cancer.
Synonyms :
MET, AUTS9, C-Met, HGF/SF receptor, HGFR, Met kinase, Proto-oncogene c-Met, Scatter factor receptor, SF receptor, HGF receptor, RCCP2, Tyrosine-protein kinase Met
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.