This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ NES / Nestin Antibody (aa1464-1614) LS-B2777
catalog :
LS-B2777
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
product information
AntibodyID :
90032
AntibodyName :
LS-B2777
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IHC-plus™ NES / Nestin Antibody (aa1464-1614) LS-B2777
Specificity :
This antibody is specific for human nestin. Species cross-reactivity: This antibody recognizes human nestin protein. It does not cross-react with rodent protein.
ClonalityDesc :
Monoclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa1464-1614
PresentationDesc :
PBS, 0.09% Sodium Azide
ImmunogenType :
Protein
ImmunogenDesc :
A fragment of the human nestin protein corresponding to residues 1464-1614.
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: store at -20°C.
IsotypeName :
IgG1,k
Gene :
NES / Nestin
StandardGeneSymbol :
NES
Reactivity :
Human
NonReactiveSpecies :
Rodent
Usage :
ICC, IF, IHC, IHC-Fr, IHC-P (5 µg/ml), WB (1:1000 - 1:5000)
ShortWebDescription :
Nestin antibody LS-B2777 is an unconjugated mouse monoclonal antibody to human Nestin (NES) (aa1464-1614). Validated for ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
UsageText :
Immunohistochemistry: LS-B2777 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2777 was determined to be 5 ug/ml. Western Blot: 1:1000-1:5000. Doublet band seen ~220-240kD.
Synonyms :
NES, Nestin, Nbla00170
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.