This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ ABL1 / c-ABL Antibody (clone 8E9., Azide-free) LS-B2776
catalog :
LS-B2776
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
8E9.

The same clone is also sold as:
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
product information
AntibodyID :
90031
AntibodyName :
LS-B2776
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IHC-plus™ ABL1 / c-ABL Antibody (clone 8E9., Azide-free) LS-B2776
Specificity :
Recognizes human c-Abl p120 and chimeric Bcr/Abl proteins. Species cross-reactivity: mouse.
ClonalityDesc :
Monoclonal
CloneName :
8E9.
AntibodyModification :
Azide-free Unconjugated
PresentationDesc :
PBS, pH 7.4
ImmunogenType :
Recombinant protein
ImmunogenDesc :
Recombinant Abl protein.
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG1
Gene :
ABL1 / c-ABL
StandardGeneSymbol :
ABL1
gene family :
Protein Kinase
Subfamily :
Abl
Reactivity :
Mouse, Human
Usage :
IF, IHC, IHC-P (10 µg/ml), IP (2 µg/tst), WB (1 µg/ml)
ShortWebDescription :
C-ABL antibody LS-B2776 is an unconjugated mouse monoclonal antibody to c-ABL (ABL1) from human. It is reactive with human and mouse. Validated for IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
UsageText :
Immunohistochemistry: LS-B2776 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2776 was determined to be 10 ug/ml. Immunoprecipitation: 2 ug/mg protein lysate. Use Protein G. Western Blot: 1 ug/ml for 2hrs at RT. Positive control: HeLa cells or placenta. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
Synonyms :
ABL1, Bcr/c-abl oncogene protein, Bcr-Abl, Bcr/abl, C-ABL, ABL, JTK7, p150, Tyrosine-protein kinase ABL1, Proto-oncogene c-Abl, V-abl
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.