IHC-plus™ ADAM12 Antibody (aa2-14) LS-B2756 | LifeSpan Biosciences LS-B2756 product information

product summary

company name :

LifeSpan Biosciences

product type :

antibody

product name :

IHC-plus™ ADAM12 Antibody (aa2-14) LS-B2756

catalog :

LS-B2756

clonality :

polyclonal

host :

domestic goat

conjugate :

nonconjugated

reactivity :

human

application :

immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

LifeSpan Biosciences product webpage

citations: 1

Reference
Kauttu T, Mustonen H, Vainionpää S, Krogerus L, Ilonen I, Rasanen J, et al. Disintegrin and metalloproteinases (ADAMs) expression in gastroesophageal reflux disease and in esophageal adenocarcinoma. Clin Transl Oncol. 2017;19:58-66 pubmed publisher

image

image 1 :

Antibody staining (0.1 ug/ml) of human heart lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

image 2 :

Anti-ADAM12 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.

image 3 :

ADAM12 antibody immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic/plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).

product information

AntibodyID :

90011

AntibodyName :

LS-B2756

TargetSpecies :

Human

Host Species :

Goat

Product Name :

IHC-plus™ ADAM12 Antibody (aa2-14) LS-B2756

Specificity :

Human ADAM12. This antibody is expected to recognise both the longer membrane-bound form of human ADAM12 (NP_003465.3) and the shorter soluble ADAM12 splice isoform (NP_067673.2). This antibody does not cross-react with other ADAMS.

ClonalityDesc :

Polyclonal

AntibodyModification :

Unconjugated

AntigenModification :

aa2-14

PresentationDesc :

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA

ImmunogenDesc :

Peptide with sequence AARPLPVSPARALC, from the N-Terminus of protein sequence according to NP_003465.3NP_067673.2.

PurificationDesc :

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

RecommendedStorageDesc :

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Gene :

ADAM12

BlockingPeptide :

LS-E25784

StandardGeneSymbol :

ADAM12

gene family :

Protease

Subfamily :

Metallopeptidase M12B

Reactivity :

Human

Usage :

IF (10 µg/ml), IHC, IHC-P (3.75 µg/ml), Peptide-ELISA (1:64000)

ShortWebDescription :

ADAM12 antibody LS-B2756 is an unconjugated goat polyclonal antibody to human ADAM12 (aa2-14). Validated for IF, IHC and Peptide-ELISA. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.

UsageText :

Immunohistochemistry: LS-B2756 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2756 was determined to be 3.75 ug/ml.

Synonyms :

ADAM12, ADAM 12, MCMPMltna, MCMP, MLTN, Meltrin-alpha, MLTNA

SalesRegion :

Worldwide

more info or order :

LifeSpan Biosciences product webpage

company information

LifeSpan Biosciences

2401 Fourth Avenue, Suite 900
Seattle, WA 98121

CustomerSupport@lsbio.com

https://www.lsbio.com

1-206-464-1554

headquarters: USA

Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.