IHC-plus™ Collagen IV Antibody LS-B2717 | LifeSpan Biosciences LS-B2717 product information

product summary

company name :

LifeSpan Biosciences

product type :

antibody

product name :

IHC-plus™ Collagen IV Antibody LS-B2717

catalog :

LS-B2717

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

LifeSpan Biosciences product webpage

citations: 1

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; human; 1:100; loading ...	Dabelsteen S, Pallesen E, Marinova I, Nielsen M, Adamopoulou M, Rømer T, et al. Essential Functions of Glycans in Human Epithelia Dissected by a CRISPR-Cas9-Engineered Human Organotypic Skin Model. Dev Cell. 2020;54:669-684.e7 pubmed publisher

image

image 1 :

Anti-Collagen IV antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.

product information

AntibodyID :

88515

AntibodyName :

LS-B2717

TargetSpecies :

Mammal

Host Species :

Rabbit

Product Name :

IHC-plus™ Collagen IV Antibody LS-B2717

Specificity :

Typically negligible cross-reactivity against other types of collagens was detected by ELISA against purified standards. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type IV collagens and has negligible cross-reactivity with Type I, II, III, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

ClonalityDesc :

Polyclonal

AntibodyModification :

Unconjugated

PresentationDesc :

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

ImmunogenType :

Purified protein

ImmunogenDesc :

Collagen Type IV from human and bovine placenta.

PurificationDesc :

Affinity chromatography

RecommendedStorageDesc :

Store vial at 4°C prior to opening. This product is stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20°C or below.

IsotypeName :

IgG

Gene :

Collagen IV

Reactivity :

Bovine, Mammal, Human

Usage :

ELISA (1:5000 - 1:50000), IHC, IHC-P (2.5 µg/ml), IP (1:100), WB (1:1000 - 1:10000)

ShortWebDescription :

Collagen IV antibody LS-B2717 is an unconjugated rabbit polyclonal antibody to Collagen IV from mammal. It is reactive with human, bovine and mammal. Validated for ELISA, IHC, IP and WB.

UsageText :

Immunohistochemistry: LS-B2717 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2717 was determined to be 2.5 ug/ml.

SalesRegion :

Worldwide

more info or order :

LifeSpan Biosciences product webpage

company information

LifeSpan Biosciences

2401 Fourth Avenue, Suite 900
Seattle, WA 98121

CustomerSupport@lsbio.com

https://www.lsbio.com

1-206-464-1554

headquarters: USA

Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.