This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ KRT19 / CK19 / Cytokeratin 19 Antibody (aa312-335, clone A53-B/A2.26) LS-B2650
catalog :
LS-B2650
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
A53-B/A2.26
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
product information
AntibodyID :
87656
AntibodyName :
LS-B2650
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IHC-plus™ KRT19 / CK19 / Cytokeratin 19 Antibody (aa312-335, clone A53-B/A2.26) LS-B2650
Specificity :
Recognizes the rod domain of keratin 19 (40kD). Reacts with the MCF-7 cells, which are known to contain keratin 19. It shows no reaction with the cells lacking keratin 19 such as A431 and HeLa. Species cross-reactivity: Human. Weak reactivity with rat, mouse and guinea pig.
ClonalityDesc :
Monoclonal
CloneName :
A53-B/A2.26
AntibodyModification :
Unconjugated
AntigenModification :
aa312-335
PresentationDesc :
10 mM PBS, pH 7.4, 0.09% Sodium Azide, 0.2% BSA
ImmunogenType :
Cells
ImmunogenDesc :
Human breast cancer MCF-7 cells. Epitope: aa312-335 Cellular Localization: Cytoplasmic. MW of antigen: 40kD
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG2a
Gene :
KRT19 / CK19 / Cytokeratin 19
StandardGeneSymbol :
KRT19
Reactivity :
Mouse, Guinea pig, Rat, Human
Usage :
IF, IHC, IHC-Fr (1 - 2 µg/ml), IHC-P (10 µg/ml), WB (2 - 4 µg/ml)
ShortWebDescription :
Cytokeratin 19 antibody LS-B2650 is an unconjugated mouse monoclonal antibody to Cytokeratin 19 (KRT19 / CK19 ) from human, mouse, rat and other species. Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
UsageText :
Immunohistochemistry: LS-B2650 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2650 was determined to be 10 ug/ml. Western Blot: 2-4 ug/ml for 2 hrs at RT. Positive control: LS174T and HT29 cells, skin.
Synonyms :
KRT19, CK19, Cytokeratin 19, Cytokeratin-19, Keratin 19, Keratin-19, CK-19, K19, K1CS, Keratin, type I, 40-kd
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
antibodies@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.