This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ ICAM-1 / CD54 Antibody LS-B2647
catalog :
LS-B2647
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
reactivity :
human
application :
immunohistochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
product information
AntibodyID :
87353
AntibodyName :
LS-B2647
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IHC-plus™ ICAM-1 / CD54 Antibody LS-B2647
Specificity :
Recognizes the human CD54 cell surface antigen, a 90kD glycoprotein also known as ICAM-1. CD54 is expressed by many cells following activation by inflammatory mediators. Clone 3H1547 blocks CD54 function.
ClonalityDesc :
Monoclonal
AntibodyModification :
Unconjugated
PresentationDesc :
PBS, pH 7.4, 0.09% Sodium Azide
ImmunogenType :
Cells
ImmunogenDesc :
Human monocytes
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG1
Gene :
ICAM-1 / CD54
StandardGeneSymbol :
ICAM1
gene family :
ICAM
Reactivity :
Human
Usage :
Flo (1:50 - 1:100), IHC, IHC-Fr (1:50 - 1:100), IHC-P (10 µg/ml), IP
ShortWebDescription :
CD54 antibody LS-B2647 is an unconjugated mouse monoclonal antibody to human CD54 (ICAM-1). Validated for Flow, IHC and IP. Tested on 20 paraffin-embedded human tissues.
UsageText :
Immunohistochemistry: LS-B2647 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2647 was determined to be 10 ug/ml. Flow Cytometry: 1:50-1:100. Use 10 ul to label 10^6 cells in 100ul. Positive control: Human Tonsil.
Synonyms :
ICAM1, BB2, CD54 antigen, CD54, ICAM-1, p3.58
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.