product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ AIF1 / IBA1 Antibody (aa149-161) LS-B2645
catalog :
LS-B2645
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, immunohistochemistry, immunohistochemistry - paraffin section
more info or order :
citations: 1
| Published Application/Species/Sample/Dilution | Reference |
|---|---|
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image
image 1 :
AIF1 / IBA1 antibody (1µg/ml) staining of Human Brain (Frontal Cortex (A) Rat (B) Mouse (C) Brain and Swine E) Brain lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
image 2 :
Anti-AIF1 / IBA1 antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
product information
AntibodyID :
87351
AntibodyName :
LS-B2645
TargetSpecies :
Human
Host Species :
Goat
Product Name :
IHC-plus™ AIF1 / IBA1 Antibody (aa149-161) LS-B2645
Specificity :
Human AIF1 / IBA1. This antibody is expected to recognize isoform 1 (NP_116573.1) and isoform 3 (NP_001614.3).
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa149-161
PresentationDesc :
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
ImmunogenDesc :
Peptide with sequence C-TGPPAKKAISELP, from the C-Terminus of protein sequence according to NP_116573.1NP_001614.3.
PurificationDesc :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
RecommendedStorageDesc :
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Gene :
AIF1 / IBA1
BlockingPeptide :
LS-E25347
StandardGeneSymbol :
AIF1
Reactivity :
Mouse, Rat, Hamster, Pig, Human, Monkey
Usage :
IHC, IHC-P (5 µg/ml), Peptide-ELISA (1:128000), WB (0.3 - 1 µg/ml)
ShortWebDescription :
IBA1 antibody LS-B2645 is an unconjugated goat polyclonal antibody to IBA1 (AIF1) (aa149-161) from human. It is reactive with human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
UsageText :
Immunohistochemistry: LS-B2645 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2645 was determined to be 5 ug/ml.
Synonyms :
AIF1, AIF-1, Em:AF129756.17, G1, IRT-1, Protein G1, IBA1, IRT1
SalesRegion :
Worldwide
more info or order :
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.
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