This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ SLC2A4 / GLUT-4 Antibody LS-B26
catalog :
LS-B26
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
immunohistochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
product information
AntibodyID :
27066
AntibodyName :
LS-B26
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ SLC2A4 / GLUT-4 Antibody LS-B26
Specificity :
Recognizes human Solute carrier family 2, facilitated glucose transporter member 4, also known as glucose transporter 4 or GLUT4. GLUT4 is a 509 amino acid ~50 kD multi-pass transmembrane protein located primarily in the perinuclear region and is translocated to the plasma membrane where it is rapidly reinternalized. Does not react with HEP G2 type glucose transporter in human erythrocytes, rat brain or other tissues that do not exhibit sensitivity to insulin. GLUT4 is the insulin-regulated glucose transporter found in adipose tissue and striated muscle, responsible for glucose disposal. The stimulation of glucose uptake by insulin requires translocation of the GLUT4 glucose transporter from intracellular storage sites to the cell surface. Activation of phosphatidylinositol-3-OH kinase (PI3K) is required for this trafficking event, but it is not sufficient to produce GLUT4 translocation. Human insulin resistance involves a defect in GLUT4 traffic and targeting leading to accumulation in a dense membrane compartment from which insulin is unable to recruit GLUT4 to the cell surface.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
PresentationDesc :
PBS, 0.09% Sodium Azide
ImmunogenType :
Synthetic peptide - KLH conjugated
ImmunogenDesc :
A 12 AA peptide corresponding to the carboxyl terminus of the insulin regulatable glucose transporter conjugated to keyhole limpet hemocyanin.
PurificationDesc :
Affinity purified
RecommendedStorageDesc :
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
IsotypeName :
IgG
Gene :
SLC2A4 / GLUT-4
StandardGeneSymbol :
SLC2A4
gene family :
Transporter
Subfamily :
Sugar transporter
Reactivity :
Human
NonReactiveSpecies :
Pig
Usage :
IHC, IHC-Fr, IHC-P (10 - 20 µg/ml)
ShortWebDescription :
GLUT-4 antibody LS-B26 is an unconjugated rabbit polyclonal antibody to human GLUT-4 (SLC2A4). Validated for IHC. Tested on 20 paraffin-embedded human tissues.
UsageText :
Immunohistochemistry: LS-B26 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B26 was determined to be 10-20 ug/ml.
Synonyms :
SLC2A4, Glucose transporter 4, Glucose transporter GLUT4, GLUT4, GLUT-4
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.