This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ RAD51 / RECA Antibody (Azide-free) LS-B2597
catalog :
LS-B2597
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section
product information
AntibodyID :
85803
AntibodyName :
LS-B2597
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IHC-plus™ RAD51 / RECA Antibody (Azide-free) LS-B2597
Specificity :
Recognizes human Rad51. Species cross-reactivity: mouse and rat.
ClonalityDesc :
Monoclonal
AntibodyModification :
Azide-free Unconjugated
PresentationDesc :
10 mM PBS, pH 7.4
ImmunogenType :
Recombinant protein
ImmunogenDesc :
Recombinant Rad51 protein. Cellular Localization: Nuclear. MW of Antigen: 37-43kD
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG1,k
Gene :
RAD51 / RECA
StandardGeneSymbol :
RAD51
Reactivity :
Mouse, Rat, Human
Usage :
IHC, IHC-P (10 µg/ml), IP, WB (1 - 2 µg/ml)
ShortWebDescription :
RECA antibody LS-B2597 is an unconjugated mouse monoclonal antibody to RECA (RAD51) from human. It is reactive with human, mouse and rat. Validated for IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
UsageText :
Immunohistochemistry: LS-B2597 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2597 was determined to be 10 ug/ml. Immunoprecipitation (Native verified): 2 ug/mg protein lysate. Use Protein G. Western Blot: 1-2 ug/ml for 2 hrs at RT. Positive control: HeLa cells and testis. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
Synonyms :
RAD51, BRCC5, HsT16930, RAD51 homolog A, RAD51 homolog (S. cerevisiae), RecA, E. coli, homolog of, RecA-like protein, Recombination protein A, MRMV2, HRAD51, HsRad51, RAD51A, RECA
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.