product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
PathPlus™ CD40 Antibody (clone LOB7/6) LS-B2596
catalog :
LS-B2596
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
LOB7/6
reactivity :
human
application :
immunohistochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - paraffin section; human
Giannopoulos K, Mertens D, Buhler A, Barth T, Idler I, Moller P, et al. The candidate immunotherapeutical target, the receptor for hyaluronic acid-mediated motility, is associated with proliferation and shows prognostic value in B-cell chronic lymphocytic leukemia. Leukemia. 2009;23:519-27 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B2596 image 1
Anti-CD40 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
product information
AntibodyID :
85802
AntibodyName :
LS-B2596
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
PathPlus™ CD40 Antibody (clone LOB7/6) LS-B2596
Specificity :
Recognizes the human CD40 cell surface antigen, a 48kD glycoprotein expressed by B lymphocytes and weakly by some monocytes. CD40 is involved in the process of B cell selection in germinal centers and is vital in T cell-B cell interactions.
ClonalityDesc :
Monoclonal
CloneName :
LOB7/6
AntibodyModification :
Unconjugated
PresentationDesc :
TBS, 0.09% Sodium Azide
ImmunogenType :
Recombinant protein
PurificationDesc :
Protein A purified from tissue culture supernatant
RecommendedStorageDesc :
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
IsotypeName :
IgG2a
Gene :
CD40
StandardGeneSymbol :
CD40
gene family :
TNF Receptor
Reactivity :
Human
Usage :
Flo (20 µg/ml), IHC, IHC-Fr (1 - 10 µg/ml), IHC-P (10 µg/ml), IP
ShortWebDescription :
CD40 antibody LS-B2596 is an unconjugated mouse monoclonal antibody to human CD40. Validated for Flow, IHC and IP. Cited in 1 publication.
UsageText :
Immunohistochemistry: LS-B2596 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2596 was determined to be 10 ug/ml. Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100ul. Immunohistochemistry: This product requires protein digestion pre-treatment of paraffin embedded sections prior to staining e. g. pronase or trypsin. Histology Positive Control Tissue: Human Tonsil.
Synonyms :
CD40, B-cell surface antigen CD40, B cell-associated molecule, B cell surface antigen CD40, CD40 antigen, CD40 type II isoform, p50, Bp50, CD40L receptor, CDW40, TNFRSF5
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.