This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ MMP9 / Gelatinase B Antibody LS-B2572
catalog :
LS-B2572
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
reactivity :
human
application :
immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
product information
AntibodyID :
85478
AntibodyName :
LS-B2572
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IHC-plus™ MMP9 / Gelatinase B Antibody LS-B2572
Specificity :
Recognizes human protein of 92kD and 78kD, under non-reducing conditions, which are identified as pro (latent) and activated forms of matrix metalloproteinase 9 (MMP-9; also known as 92kD collagenase IV or gelatinase B). Also reacts with the complex 200kD) of MMP-9 and tissue inhibitor of metalloproteinases-1 TIMP-1). Shows no cross-reactivity with pro and active forms of MMP-2.
ClonalityDesc :
Monoclonal
AntibodyModification :
Unconjugated
PresentationDesc :
PBS, pH 7.4, 0.09% Sodium Azide, 40% Glycerol, 0.2% BSA
ImmunogenDesc :
Purified human MMP-9. Cellular Localization: Cytoplasmic. MW of antigen: 92kD (pro form) and ~86kD (active form).
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG1
Gene :
MMP9 / Gelatinase B
StandardGeneSymbol :
MMP9
gene family :
Protease
Subfamily :
Metallopeptidase M10A
Reactivity :
Human
Usage :
ELISA, IHC, IHC-Fr, IHC-P (10 µg/ml), IP
ShortWebDescription :
Gelatinase B antibody LS-B2572 is an unconjugated mouse monoclonal antibody to human Gelatinase B (MMP9). Validated for ELISA, IHC and IP. Tested on 20 paraffin-embedded human tissues.
UsageText :
Immunohistochemistry: LS-B2572 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2572 was determined to be 10 ug/ml. Inhibition of MMP-9 Activity: 2-4 ug/ml (order Ab without sodium azide). Immunoprecipitation (Protein G): 2 ug/mg protein lysate (Native verified). Positive control: Conditioned, serum-free medium from (TPA-treated) human fibrosarcoma HT-1080 cells.
Synonyms :
MMP9, 92kd gelatinase, 92 kDa gelatinase, Matrix metalloproteinase-9, MMP-9, CLG4B, Gelatinase B, GELB, Macrophage gelatinase, MANDP2
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.