This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ F2 / Prothrombin / Thrombin Antibody LS-B2559
catalog :
LS-B2559
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section
product information
AntibodyID :
85034
AntibodyName :
LS-B2559
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IHC-plus™ F2 / Prothrombin / Thrombin Antibody LS-B2559
Specificity :
Recognizes human Prothrombin, prethrombin-1, fragment 2, meizothrombin. Inhibits clotting and prothrombin activation. Epitope: Located in the fragment 2 region (aa156-271)
ClonalityDesc :
Monoclonal
AntibodyModification :
Unconjugated
PresentationDesc :
Supplied as a liquid in 50% glycerol, ddH2O. Sourced from ascites.
ImmunogenType :
Purified protein
ImmunogenDesc :
Human Prothrombin.
PurificationDesc :
Ion exchange chromatography
RecommendedStorageDesc :
Short term: 4°C Long term: Aliquot and store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG2a
Gene :
F2 / Prothrombin / Thrombin
StandardGeneSymbol :
F2
gene family :
Protease
Subfamily :
Serine S1
Reactivity :
Human
Usage :
ELISA (5 - 10 µg/ml), IHC, IHC-P (5 µg/ml), WB (10 µg/ml)
ShortWebDescription :
Thrombin antibody LS-B2559 is an unconjugated mouse monoclonal antibody to human Thrombin (F2 / Prothrombin). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
UsageText :
Immunohistochemistry: LS-B2559 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2559 was determined to be 5 ug/ml.
Synonyms :
F2, Prothrombin B-chain, Coagulation factor II, FACTOR II, Prothrombin, PT, RPRGL2, Serine protease, THPH1, Thrombin
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.